Borevitz Justin
Ecology and Evolution, University of Chicago, IL, USA.
Methods Mol Biol. 2006;323:137-45. doi: 10.1385/1-59745-003-0:137.
This chapter discusses the specific details of how oligonucleotide arrays originally designed for expression analysis can be used for high-density genotyping to reveal tens of thousands of single-feature polymorphisms (SFPs). Cluster of SFPs reveal potential natural deletion polymorphisms. SFPs can be readily used for mapping mutations using bulk segregant analysis. An example is given using the Columbia and Landsberg erecta strains of Arabidopsis thaliana.
本章讨论了最初设计用于表达分析的寡核苷酸阵列如何用于高密度基因分型以揭示数以万计的单特征多态性（SFP）的具体细节。SFP簇揭示了潜在的自然缺失多态性。SFP可通过混合分离群体分析法轻松用于突变定位。文中给出了一个使用拟南芥哥伦比亚和直立型兰茨贝格品系的示例。
