Bodart Jean-François L, Duesbery Nicholas S
Laboratoire de Biologie du Développement, UPRES EA3, Université des sciences et technologies de Lille, France.
Methods Mol Biol. 2006;322:43-53. doi: 10.1007/978-1-59745-000-3_4.
For more than 30 yr, Xenopus laevis has been the animal of choice for studying the biochemical regulation of the meiotic and early mitotic vertebrate cell cycles. Attracted by its diploid genome, several laboratories have begun using the similar, although evolutionarily distinct, frog Xenopus tropicalis for studies of vertebrate development. Comparisons between the two species indicate that their development is similar in most respects. Both frogs share many advantages, including their amenability to manipulation and their ability to produce large numbers of high-quality oocytes and eggs year round. In addition, X. tropicalis possesses several advantages that, when combined with its potential for genetic studies, makes it an attractive, complementary model for vertebrate developmental biology. In this chapter, we note some of these advantages and describe in detail techniques we have adapted for the study of meiosis in X. tropicalis.
30多年来，非洲爪蟾一直是研究减数分裂和早期有丝分裂脊椎动物细胞周期生化调控的首选动物。受其二倍体基因组的吸引，一些实验室已开始使用亲缘关系相近但在进化上有差异的热带爪蟾来研究脊椎动物的发育。对这两个物种的比较表明，它们在大多数方面的发育是相似的。这两种爪蟾都有许多优点，包括易于操作以及全年都能产出大量高质量的卵母细胞和卵子。此外，热带爪蟾还具有一些优势，再加上其在遗传学研究方面的潜力，使其成为脊椎动物发育生物学中一个有吸引力的互补模型。在本章中，我们将指出其中一些优势，并详细描述我们为研究热带爪蟾减数分裂而采用的技术。
