Bahia D, Avelar L G A, Vigorosi F, Cioli D, Oliveira G C, Mortara R A
Laboratório de Parasitologia Celular e Molecular, Centro de Pesquisas René Rachou-FIOCRUZ, Av. Augusto de Lima 1715, 30190-002, Belo Horizonte, MG, Brazil.
Parasitology. 2006 Sep;133(Pt 3):321-9. doi: 10.1017/S0031182006000400. Epub 2006 Jun 2.
Schistosoma mansoni eggs, miracidia and primary sporocysts were labelled with phalloidin-rhodamine to visualize filamentous actin structures. Analysis of these forms by confocal fluorescence microscopy revealed the presence of previously well-defined circular and longitudinal muscle layers. Besides these muscular layers that sustain and provide motility to these parasite forms, we found in these 3 consecutive developmental stages of the parasite previously unidentified actin-rich tubular structures. In the 3 forms, 4 actin-rich tubules could be observed by optical sectioning underneath the well-developed muscle layers. The tubules appear in pairs, transversal to the length of the parasite, and located towards the extremities. By using an anti-flame cell specific antibody we confirmed that the tubules co-localize with flame cells and also determined that the tubule core is filled with microtubules. The additional presence of myosin in these tubules strongly suggests that they are contractile structures.
曼氏血吸虫卵、毛蚴和初级孢蚴用罗丹明鬼笔环肽标记，以观察丝状肌动蛋白结构。通过共聚焦荧光显微镜对这些形态进行分析，发现了先前明确的环形和纵向肌肉层。除了这些维持并为这些寄生虫形态提供运动能力的肌肉层外，我们在寄生虫的这3个连续发育阶段中发现了以前未识别的富含肌动蛋白的管状结构。在这3种形态中，通过光学切片在发育良好的肌肉层下方可观察到4个富含肌动蛋白的小管。这些小管成对出现，与寄生虫的长度方向横向，且位于两端。通过使用抗焰细胞特异性抗体，我们证实这些小管与焰细胞共定位，还确定小管核心充满微管。这些小管中额外存在肌球蛋白，强烈表明它们是收缩结构。
