Identification of a natriuretic peptide (NP) in cyclostomes (lamprey and hagfish): CNP-4 is the ancestral gene of the NP family.

In bony fishes, natriuretic peptides (NPs) comprise a hormone family that is composed of seven subtypes; ANP, BNP, VNP that have an intramolecular ring and N- and C-terminal extensions, and four CNPs (CNP-1 to -4) that lack the C-terminal extension. To assess the ancestral molecule of the NP family, we determined the NP sequences in several species of two extant cyclosotome groups, lampreys and hagfishes. A cDNA encoding CNP was cloned from the heart and brain of three phylogenetically distant species of lampreys, Geotria australis, Lampetra japonica, and Petromyzon marinus. In the deduced prohormone sequence of each species, two potential processing signals, lysine-lysine (KK) that is commonly present in CNP precursors, and arginine-X-X-arginine (RXXR) for furin-like proprotein convertase (PC) that is typical for CNP-4 were present. The deduced mature peptides that are released at each signal were highly conserved among three species; 100% cleaved at KK and >92% processed at RXXR. In L. japonica, the CNP gene was expressed almost exclusively in the heart and brain. Meanwhile, a cDNA encoding NP with a C-terminal tail sequence was cloned from the heart and brain of three hagfish species in different genera, Myxine glutinosa, Eptatretus cirrhatus, and Paramyxine atami. The precursor sequences including the prosegment had >80% identity among the three hagfish species. A processing signal, RXXR, is also conserved in the prosegment of all hagfish NPs. The molecular phylogenetic analyses inferred that the lamprey CNP and hagfish NP belong to the CNP-4 group, even though the hagfish NP has a C-terminal sequence extended from the intramolecular ring. The presence of a processing signal, RXXR, in the prosegment of cyclostome NPs supports the above classification. Based on the current findings, we suggest that the ancestral gene of the NP family is CNP-4.