从大肠杆菌的dnaA启动子中消除多个GATC位点后，同步化表型仍然存在。
The synchrony phenotype persists after elimination of multiple GATC sites from the dnaA promoter of Escherichia coli.
作者信息
Wilkinson Terry G, Kedar G C, Lee Chi, Guzmán Elena C, Smith Douglas W, Zyskind Judith W
机构信息
Department of Biology, San Diego State University, 5500 Campanile Drive, San Diego, CA 92182-4614, USA.
出版信息
J Bacteriol. 2006 Jun;188(12):4573-6. doi: 10.1128/JB.00089-06.
Abstract
To examine whether methylation of the GATC sites present in the dnaA promoter region is responsible for the strict temporal coordination of initiation events at oriC as measured by the synchrony of initiation, we introduced point mutations eliminating three (TGW1) and five (TGW2) of the six GATC sites present in the dnaA promoter region. All of the strains containing these mutations, including the one with five GATC sites eliminated, initiated chromosomal replication synchronously.
摘要
为了检验dnaA启动子区域中存在的GATC位点的甲基化是否如通过起始同步性所测量的那样，负责oriC处起始事件的严格时间协调，我们引入了点突变，消除了dnaA启动子区域中六个GATC位点中的三个（TGW1）和五个（TGW2）。所有含有这些突变的菌株，包括消除了五个GATC位点的菌株，都能同步启动染色体复制。
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