蛋白水解酶催化的动力学和机制：牛胰蛋白酶对 l-赖氨酸和 S-(β-氨基乙基)-l-半胱氨酸（硫代赖氨酸）衍生物的水解动力学。

Department of Biochemistry, Queen's University, Belfast, N. Ireland.

Biochem J. 1967 Mar;102(3):728-34. doi: 10.1042/bj1020728.

Several esters of the alpha-N-toluene-p-sulphonyl and N-benzoyl derivatives of l-lysine and S-(beta-aminoethyl)-l-cysteine have been synthesized. 2. The kinetics of hydrolysis of the esters by bovine trypsin have been compared. Values of k(0) are similar for corresponding derivatives of the isosteric amino acids and deacylation of an acyl-enzyme appears to be rate-determining in each case. There are, however, some quantitative kinetic differences between the various series of substrates.

已合成了 l-赖氨酸和 S-（β-氨基乙基）-l-半胱氨酸的α-N-对甲苯磺酰基和 N-苯甲酰基衍生物的几种酯。
比较了牛胰蛋白酶对酯的水解动力学。同系氨基酸的相应衍生物的 k(0)值相似，并且在每种情况下，脱酰基似乎是酰化酶的速率决定步骤。然而，各种系列底物之间存在一些定量动力学差异。

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核心技术专利：CN118964589B侵权必究

Department of Biochemistry, Queen's University, Belfast, N. Ireland.

Biochem J. 1967 Mar;102(3):728-34. doi: 10.1042/bj1020728.

Several esters of the alpha-N-toluene-p-sulphonyl and N-benzoyl derivatives of l-lysine and S-(beta-aminoethyl)-l-cysteine have been synthesized. 2. The kinetics of hydrolysis of the esters by bovine trypsin have been compared. Values of k(0) are similar for corresponding derivatives of the isosteric amino acids and deacylation of an acyl-enzyme appears to be rate-determining in each case. There are, however, some quantitative kinetic differences between the various series of substrates.

已合成了 l-赖氨酸和 S-（β-氨基乙基）-l-半胱氨酸的α-N-对甲苯磺酰基和 N-苯甲酰基衍生物的几种酯。
比较了牛胰蛋白酶对酯的水解动力学。同系氨基酸的相应衍生物的 k(0)值相似，并且在每种情况下，脱酰基似乎是酰化酶的速率决定步骤。然而，各种系列底物之间存在一些定量动力学差异。