Girardi G, Elias M M
Farmacologia, Facultad de Ciencias Bioquimicas y Farmaceuticas, Universidad Nacional de Rosario, Argentina.
Toxicology. 1991 Apr 8;67(2):155-64. doi: 10.1016/0300-483x(91)90139-r.
Mercuric chloride (HgCl2)-induced nephrotoxicity, as measured by functional and biochemical parameters was evaluated in rats at different kidney non-protein sulfhydryls (NPS) levels. Diethylmaleate (DEM) induced a 75% of NPS diminution 1 h after the administration. Renal function (clearance) and biochemical measurements (gamma-glutamyltranspeptidase activity in urine, and lipoperoxides in kidney tissue) were impaired when the animals were HgCl2-treated. Values were highly impaired when the kidneys were NPS-depleted and were improved when NPS pools were previously increased although they were not similar to control values. DEM treatment promoted a higher accumulation of HgCl2 in both kidney and liver while NAC-treatment reduced significantly the metal content in these organs. These data are in favour of a positive relationship among mercury content and organ injury. On the other hand, mercury content increased while NPS levels diminished. NPS might play a role in the HgCl2 detoxification and thus avoids mercury accumulation and mercury effects.
通过功能和生化参数测定，在不同肾脏非蛋白巯基（NPS）水平的大鼠中评估了氯化汞（HgCl₂）诱导的肾毒性。马来酸二乙酯（DEM）给药1小时后可使NPS减少75%。当动物接受HgCl₂处理时，肾功能（清除率）和生化指标（尿γ-谷氨酰转肽酶活性和肾组织中的脂质过氧化物）受损。当肾脏NPS耗竭时，各项指标严重受损；而当NPS储备预先增加时，指标有所改善，尽管仍与对照值不同。DEM处理促进了HgCl₂在肾脏和肝脏中的更高积累，而N-乙酰半胱氨酸（NAC）处理则显著降低了这些器官中的金属含量。这些数据支持汞含量与器官损伤之间存在正相关关系。另一方面，汞含量增加而NPS水平降低。NPS可能在HgCl₂解毒中发挥作用，从而避免汞的积累和汞的影响。
