Wakefield A E, Guiver L, Miller R F, Hopkin J M
Department of Paediatrics, John Radcliffe Hospital, Oxford, UK.
Lancet. 1991 Jun 8;337(8754):1378-9. doi: 10.1016/0140-6736(91)93062-e.
DNA amplification and silver staining were used to identify Pneumocystis carinii in bronchoscopic lavage and induced sputum samples during 51 episodes of respiratory illness in 47 subjects with HIV infection. In 20 episodes, in which the clinical diagnosis was pneumocystis pneumonia (PCP), silver stain was positive in 14 lavage samples (70%) and 7 sputum samples (35%), whereas DNA amplification was positive in 19 lavage samples (95%) and 18 sputum samples (90%). DNA amplification was positive in only 1 of 31 patients without PCP (PCP developed in this patient within 10 weeks). DNA amplification on induced sputum offers a powerful technique for diagnosis of PCP.
在47例感染HIV的受试者出现51次呼吸道疾病发作期间，采用DNA扩增和银染色法对支气管灌洗和诱导痰样本中的卡氏肺孢子虫进行鉴定。在20例临床诊断为肺孢子虫肺炎（PCP）的病例中，14份灌洗样本（70%）和7份痰样本（35%）的银染色呈阳性，而19份灌洗样本（95%）和18份痰样本（90%）的DNA扩增呈阳性。在31例非PCP患者中，只有1例DNA扩增呈阳性（该患者在10周内发展为PCP）。诱导痰的DNA扩增为PCP的诊断提供了一种强大的技术。
