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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Department of Chemical Pathology, Institute of Neurology, The National Hospital, Queen Square, London, W.C.1.

Biochem J. 1965 Oct;97(1):151-7. doi: 10.1042/bj0970151.

The acid decolorization of caeruloplasmin was studied at various temperatures and pH values. 2. Two decolorization reactions may be distinguished: (i) an irreversible reaction with the thermodynamic characteristics of a protein denaturation; (ii) the attainment of an equilibrium between blue and colourless forms of caeruloplasmin with apparent pK 3.7 at 25 degrees . 3. The low temperature-dependence of the equilibrium suggests that it does not involve a gross denaturative change. 4. The reversible decolorization occurred both aerobically and anaerobically, indicating that the change does not involve dissociation of a copper-oxygen complex. 5. Spectral changes during the decolorization are described. 6. The changes occurring during acid decolorization are discussed in relation to a formal model of the gross structure of caeruloplasmin.

在不同温度和pH值条件下研究了铜蓝蛋白的酸脱色作用。2. 可区分出两种脱色反应：(i) 具有蛋白质变性热力学特征的不可逆反应；(ii) 在25摄氏度时铜蓝蛋白的蓝色和无色形式之间达到平衡，表观pK为3.7。3. 平衡的低温依赖性表明它不涉及总体变性变化。4. 可逆脱色在有氧和无氧条件下均会发生，表明该变化不涉及铜-氧复合物的解离。5. 描述了脱色过程中的光谱变化。6. 结合铜蓝蛋白总体结构的形式模型讨论了酸脱色过程中发生的变化。

Department of Chemical Pathology, Institute of Neurology, The National Hospital, Queen Square, London, W.C.1.

Biochem J. 1965 Oct;97(1):151-7. doi: 10.1042/bj0970151.

The acid decolorization of caeruloplasmin was studied at various temperatures and pH values. 2. Two decolorization reactions may be distinguished: (i) an irreversible reaction with the thermodynamic characteristics of a protein denaturation; (ii) the attainment of an equilibrium between blue and colourless forms of caeruloplasmin with apparent pK 3.7 at 25 degrees . 3. The low temperature-dependence of the equilibrium suggests that it does not involve a gross denaturative change. 4. The reversible decolorization occurred both aerobically and anaerobically, indicating that the change does not involve dissociation of a copper-oxygen complex. 5. Spectral changes during the decolorization are described. 6. The changes occurring during acid decolorization are discussed in relation to a formal model of the gross structure of caeruloplasmin.

在不同温度和pH值条件下研究了铜蓝蛋白的酸脱色作用。2. 可区分出两种脱色反应：(i) 具有蛋白质变性热力学特征的不可逆反应；(ii) 在25摄氏度时铜蓝蛋白的蓝色和无色形式之间达到平衡，表观pK为3.7。3. 平衡的低温依赖性表明它不涉及总体变性变化。4. 可逆脱色在有氧和无氧条件下均会发生，表明该变化不涉及铜-氧复合物的解离。5. 描述了脱色过程中的光谱变化。6. 结合铜蓝蛋白总体结构的形式模型讨论了酸脱色过程中发生的变化。