骨酶研究。骨组织中酸性水解酶及其他酶的测定。
Studies on bone enzymes. The assay of acid hydrolases and other enzymes in bone tissue.
作者信息
Vaes G, Jacques P
机构信息
Department of Physiological Chemistry, University of Louvain, Belgium.
出版信息
Biochem J. 1965 Nov;97(2):380-8. doi: 10.1042/bj0970380.
Abstract
- Nine acid hydrolases, cytochrome oxidase, alkaline phenylphosphatase and catalase were demonstrated in 0.25m-sucrose homogenates of newborn-rat calvaria. The acid hydrolases were: acid phenylphosphatase, acid beta-glycerophosphatase, beta-glucuronidase, beta-N-acetylglucosaminidase (beta-N-acetylaminodeoxyglucosidase), acid ribonuclease and acid deoxyribonuclease, showing optimum activity at about pH5; cathepsin, beta-galactosidase and hyaluronidase, with optimum activity at about pH3.6. 2. The main kinetic characters of these enzymes have been studied and methods for their quantitative assay have been worked out. The activities present in bone are given and compared with those found in liver. 3. Acid-phosphatase activity was assayed with phenyl phosphate and beta-glycerophosphate as substrates: activities with these two substrates appeared to be due to two different enzymes. Acid phenylphosphatase is particularly labile and is readily inactivated by various physical or chemical agents.
摘要
- 在新生大鼠颅骨的0.25m蔗糖匀浆中证实了9种酸性水解酶、细胞色素氧化酶、碱性苯磷酸酶和过氧化氢酶。酸性水解酶包括：酸性苯磷酸酶、酸性β-甘油磷酸酶、β-葡萄糖醛酸酶、β-N-乙酰氨基葡萄糖苷酶（β-N-乙酰氨基脱氧葡萄糖苷酶）、酸性核糖核酸酶和酸性脱氧核糖核酸酶，在pH约为5时显示最佳活性；组织蛋白酶、β-半乳糖苷酶和透明质酸酶，在pH约为3.6时具有最佳活性。2. 研究了这些酶的主要动力学特性，并制定了它们的定量测定方法。给出了骨骼中存在的活性，并与肝脏中的活性进行了比较。3. 以苯磷酸和β-甘油磷酸为底物测定酸性磷酸酶活性：这两种底物的活性似乎归因于两种不同的酶。酸性苯磷酸酶特别不稳定，容易被各种物理或化学试剂灭活。
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本文引用的文献
1
Studies on bone enzymes. The activation and release of latent acid hydrolases and catalase in bone-tissue homogenates.骨酶研究。骨组织匀浆中潜在酸性水解酶和过氧化氢酶的激活与释放。
Biochem J. 1965 Nov;97(2):393-402. doi: 10.1042/bj0970393.
2
Studies on bone enzymes. Distribution of acid hydrolases, alkaline phenylphosphatase, cytochrome oxidase and catalase in subcellular fraction of bone tissue homogenates.骨酶研究。酸性水解酶、碱性苯磷酸酶、细胞色素氧化酶和过氧化氢酶在骨组织匀浆亚细胞组分中的分布。
Biochem J. 1965 Nov;97(2):389-92. doi: 10.1042/bj0970389.
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A submicrodetermination of glucose.葡萄糖的亚微量测定
J Biol Chem. 1949 Nov;181(1):149-51.
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
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A microspectrophotometric method for the determination of cytochrome oxidase.一种测定细胞色素氧化酶的显微分光光度法。
J Biol Chem. 1951 Apr;189(2):665-70.
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Concerning pH gradients between the extracellular compartment and fluids bathing the bone mineral surface and their relation to calcium ion distribution.关于细胞外液与骨矿物质表面浸润液之间的pH梯度及其与钙离子分布的关系。
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