人类和兔γ球蛋白的二硫键
The disulphide bonds of human and rabbit gamma-globulins.
作者信息
Cecil R, Stevenson G T
机构信息
Department of Biochemistry, University of Oxford.
出版信息
Biochem J. 1965 Nov;97(2):569-72. doi: 10.1042/bj0970569.
Abstract
- The reaction of the disulphide bonds of the predominant species of human and rabbit gamma-globulins (the 7s gamma-globulins) with sulphite was studied in the presence and absence of denaturing agents and heavy-metal reagents. 2. The total number of bonds reacting/mol. of mol.wt. 160000 was approx. 18 for human and 20 for rabbit gamma-globulin. 3. Six S.S bonds/mol. of human and 6.5 S.S bonds/mol. of rabbit gamma-globulin reacted with sulphite alone at pH6. These appeared to include all the interchain S.S bonds. 4. The number of free SH groups was less than 0.2/mol. of human and less than 0.3/mol. of rabbit gamma-globulin.
摘要
- 在有和没有变性剂及重金属试剂存在的情况下，研究了人及兔γ-球蛋白的主要种类（7Sγ-球蛋白）的二硫键与亚硫酸盐的反应。2. 每摩尔分子量为160000的γ-球蛋白反应的键总数，人γ-球蛋白约为18个，兔γ-球蛋白约为20个。3. 在pH6时，单独与亚硫酸盐反应的人γ-球蛋白每摩尔有6个二硫键，兔γ-球蛋白每摩尔有6.5个二硫键。这些似乎包括了所有的链间二硫键。4. 人γ-球蛋白每摩尔游离巯基的数量少于0.2个，兔γ-球蛋白每摩尔少于0.3个。
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