Ye Xin-hua, Jin Yu, Fang Zhao-yin, Sun Ya-ping, Xie Hua-ping, Zhang Qing, Xi Jiang, Steele Duncan, Glass Roger
Department of Pediatrics, The First Hospital, Lanzhou University, Lanzhou 730000, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2006 Feb;27(2):117-22.
To study molecular epidemiology of four major etiological viruses among children with acute diarrhea in Lanzhou, Gansu province.
Stool specimens were collected from all 400 inpatients less than 5 years old with acute diarrhea admitted in Department of Pediatrics, the First Hospital, Lanzhou University from Jul. 2004 through Jun. 2005. Dako IDEIA kits were used for detection of rotavirus, adenovirus and astrovirus. Calicivirus detection was done by both reverse transcription-polymerase chain reactions (RT-PCR) and ELISA. Further strain characterization of rotavirus and astrovirus was carried out with RT-PCR.
A total of 400 stool samples were collected and tested. Rotavirus was present in 47.3% of the cases: calicivirus in 15.5%, astrovirus in 9.5% and adenoviruses in 7.5%. Mixed infections with multiple enteric viruses were present in 13.5% of all samples. Among 189 rotavirus positive samples, serotype G2 (34.4%) was the predominant strain followed by G3 (32.8%), G1 (1.1%) and mixed-G infection (5.8%). 25.9% of strains remained to be non-typeable. P genotyping showed P [4] (45%) was most common followed by P [8] (22.1%), and non-typeable (32.9%). Strain P [4] G2 (43.6%) was the most common combination followed by P [8] G3 (25.6%), P [4] G3 (13.8%), and P [8] G2, P [4] G1 and P [8] G1. Among 38 astrovirus strains, serotypes 1 (57.8%) was the predominant. Serotype 3 and 8 were found only in one case respectively, 14 strains remained to be non-typeable. A peak admission of rotavirus diarrhea was observed from October through December. More than 95.0% of viral diarrhea patients under hospitalization occurred among children younger than 2 years. The incidence rates of rotavirus were highest in infants aged 6 - 23 months.
Rotavirus was the most important pathogen for viral diarrhea among children hospitalized in Lanzhou followed by calicivirus, astrovirus, and adenovirus. The predominant rotavirus strain circulated was P [4] G2, which was different from the finding in the previous years. The high rate of mixed infection with different viral agents was notable.
研究甘肃省兰州市急性腹泻儿童中四种主要病原性病毒的分子流行病学。
收集2004年7月至2005年6月期间兰州大学第一医院儿科收治的400例5岁以下急性腹泻住院患儿的粪便标本。采用达科IDEIA试剂盒检测轮状病毒、腺病毒和星状病毒。采用逆转录-聚合酶链反应（RT-PCR）和酶联免疫吸附测定（ELISA）检测杯状病毒。采用RT-PCR对轮状病毒和星状病毒进行进一步的毒株鉴定。
共收集并检测了400份粪便样本。47.3%的病例检出轮状病毒；杯状病毒检出率为15.5%，星状病毒为9.5%，腺病毒为7.5%。13.5%的样本存在多种肠道病毒混合感染。在189份轮状病毒阳性样本中，G2型（34.4%）为主要毒株，其次是G3型（32.8%）、G1型（1.1%）和G型混合感染（5.8%）。25.9%的毒株仍无法分型。P基因型显示，P[4]（45%）最为常见，其次是P[8]（22.1%），无法分型的占32.9%。P[4]G2型毒株（43.6%）是最常见的组合，其次是P[8]G3型（25.6%）、P[4]G3型（13.8%），以及P[8]G2型、P[4]G1型和P[8]G1型。在38株星状病毒毒株中，1型（57.8%）为主要血清型。3型和8型仅分别在1例中发现，14株仍无法分型。观察到10月至12月轮状病毒腹泻入院高峰。住院的病毒性腹泻患者中，超过95.0%发生在2岁以下儿童。轮状病毒发病率在6至23个月龄婴儿中最高。
在兰州住院儿童中，轮状病毒是病毒性腹泻最重要的病原体，其次是杯状病毒、星状病毒和腺病毒。流行的主要轮状病毒毒株是P[4]G2型，与前几年的发现不同。不同病毒病原体的混合感染率较高，值得关注。
