Hampf Mathias, Gossen Manfred
Max Delbrück Center for Molecular Medicine, 13125 Berlin, Germany.
Anal Biochem. 2006 Sep 1;356(1):94-9. doi: 10.1016/j.ab.2006.04.046. Epub 2006 May 12.
We established a quantitative reporter gene protocol, the P/Rluc assay system, allowing the sequential measurement of Photinus and Renilla luciferase activities from the same extract. Other than comparable commercial reporter assay systems and their noncommercial counterparts, the P/Rluc assay system was formulated under the aspect of full compatibility with standard methods for protein assays. This feature greatly expands the range of applications for assay systems quantifying the expression of multiple luciferase reporters.
我们建立了一种定量报告基因方案，即P/Rluc检测系统，可对同一提取物中的萤火虫荧光素酶和海肾荧光素酶活性进行连续测量。除了类似的商业报告基因检测系统及其非商业同类产品外，P/Rluc检测系统是在与蛋白质检测标准方法完全兼容的前提下制定的。这一特性极大地扩展了用于定量多种荧光素酶报告基因表达的检测系统的应用范围。
