Luo Ning, Zhang Chun, Hirt Douglas E, Husson Scott M
Department of Chemical and Biomolecular Engineering and the Center for Advanced Engineering Fibers and Films, Clemson University, Clemson, SC 29634-0909, United States.
Colloids Surf B Biointerfaces. 2006 Jul 1;50(2):89-96. doi: 10.1016/j.colsurfb.2006.04.007. Epub 2006 Apr 28.
Poly(ethylene-co-acrylic acid) (EAA) films were reacted with glycine, 12-aminododecanoic acid, aspartic acid, 5-aminoisophthalic acid, ethanolamine, diethylamine, dimethylamine, N-isopropylamine, and dimethylaminoethyleneamine to prepare EAA films with negatively charged, non-charged, hydrophilic, and hydrophobic functionalities. Attenuated total reflectance Fourier transform infrared spectroscopy, differential scanning calorimetry, and contact angle measurements were used to characterize the modified EAA films. Analyses revealed that the films were modified on the surfaces and also in the bulk; therefore, bulk properties such as cohesive energy density were changed even though the surfaces remained hydrophobic. Adsorption studies were performed for two fluorescently labeled protein residues, dansyl-L-phenylalanine (dansyl-F) and dansyl-L-glutamine (dansyl-Q), from pH 7.4 buffer solutions. The adsorption results revealed that dimethylaminoethyleneamine functionality gave the highest uptake among the functionalities studied, and adsorption was more favorable for dansyl-F than dansyl-Q. Adsorption behavior is discussed in terms of hydrophobic-hydrophobic (dispersion) interactions and Coulombic interactions.
将聚（乙烯-共-丙烯酸）（EAA）薄膜与甘氨酸、12-氨基十二烷酸、天冬氨酸、5-氨基间苯二甲酸、乙醇胺、二乙胺、二甲胺、N-异丙胺和二甲基氨基乙胺反应，制备出具有带负电荷、不带电荷、亲水性和疏水性官能团的EAA薄膜。采用衰减全反射傅里叶变换红外光谱、差示扫描量热法和接触角测量对改性EAA薄膜进行表征。分析表明，薄膜不仅在表面而且在本体中都发生了改性；因此，即使表面保持疏水性，内聚能密度等本体性质也发生了变化。对来自pH 7.4缓冲溶液中的两种荧光标记蛋白质残基丹磺酰-L-苯丙氨酸（丹磺酰-F）和丹磺酰-L-谷氨酰胺（丹磺酰-Q）进行了吸附研究。吸附结果表明，在所研究的官能团中，二甲基氨基乙胺官能团的吸附量最高，且丹磺酰-F的吸附比丹磺酰-Q更有利。从疏水-疏水（色散）相互作用和库仑相互作用的角度讨论了吸附行为。
