与猪相关的非典型加拿大幽门螺杆菌菌株。
Atypical Helicobacter canadensis strains associated with swine.
作者信息
Inglis G Douglas, McConville Malcolm, de Jong Anno
机构信息
Food Safety and Quality National Program, Agriculture and Agri-Food Canada Research Centre, Alberta, T1J 4B1, Canada.
出版信息
Appl Environ Microbiol. 2006 Jun;72(6):4464-71. doi: 10.1128/AEM.02843-05.
Forty-two Helicobacter isolates were isolated from swine feces in The Netherlands and Denmark. All 12 isolates sequenced (16S rRNA gene) formed a robust clade with Helicobacter canadensis ( approximately 99% similarity). Species-specific PCR indicated that all of the isolates were H. canadensis isolates. Although the appearance of the porcine isolates was similar to the appearance of H. canadensis, only one of these isolates was able to hydrolyze indoxyl acetate, a cardinal characteristic of this taxon. Examination of the 23S rRNA and hsp60 genes revealed high levels of similarity between the porcine isolates and H. canadensis. However, amplified fragment length polymorphism genomic typing showed that isolates recovered from swine feces were genetically distinct from H. canadensis strains obtained from humans and geese.
在荷兰和丹麦，从猪粪便中分离出了42株幽门螺杆菌。所有测序的12株分离株（16S rRNA基因）与加拿大幽门螺杆菌形成了一个稳定的进化枝（相似度约为99%）。种特异性PCR表明，所有分离株均为加拿大幽门螺杆菌分离株。尽管猪分离株的外观与加拿大幽门螺杆菌相似，但这些分离株中只有一株能够水解醋酸吲哚酚，这是该分类单元的一个主要特征。对23S rRNA和hsp60基因的检测显示，猪分离株与加拿大幽门螺杆菌之间具有高度相似性。然而，扩增片段长度多态性基因组分型显示，从猪粪便中分离出的分离株在基因上与从人类和鹅中获得的加拿大幽门螺杆菌菌株不同。
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