Kasumi Hiroyuki, Komori Shinji, Sakata Kazuko, Yamamoto Naoko, Yamasaki Tomohiko, Kanemura Yonehiro, Koyama Koji
Department of Obstetrics and Gynecology, Hyogo College of Medicine, 1-1 mukogawa-cho, Nishinomiya 663-8501, Japan.
Asian J Androl. 2006 Sep;8(5):549-54. doi: 10.1111/j.1745-7262.2006.00196.x. Epub 2006 Jun 5.
To identify proteins induced by androgen in Sertoli cells during spermatogenesis.
We analyzed protein profiles in TM4 Sertoli cells treated with dihydrotestosterone (DHT) using surface enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF-MS).
We found increases in the expression of a 5.0-kDa protein at 15 min, an 11.3-kDa protein at 24 h and 4.3 kDa, 5.7 kDa, 5.8 kDa, 9.95 kDa and 9.98 kDa proteins at 48 h after the treatment. In contrast, the expression of 6.3 kDa and 8.6 kDa proteins decreased at 30 min, and 4.9 kDa, 5.0 kDa, 12.4 kDa and 19.8 kDa proteins at 48 h after the treatment. The 11.3-kDa protein was identified as macrophage migration inhibitory factor (MIF) known to having various functions. The 9.98-kDa protein was identified as calgizzarin related to calcium channels. The timing of their expression suggests that MIF and calgizzarin are involved in late regulation of spermatogenesis in Sertoli cells by androgen.
MIF and calgizzarin are two important androgen-responsive proteins produced by Sertoli cells and they might play a role in regulating spermatogenesis.
鉴定生精过程中支持细胞中雄激素诱导的蛋白质。
我们使用表面增强激光解吸电离飞行时间质谱（SELDI-TOF-MS）分析了用二氢睾酮（DHT）处理的TM4支持细胞中的蛋白质谱。
我们发现处理后15分钟时5.0 kDa蛋白质的表达增加，24小时时11.3 kDa蛋白质的表达增加，48小时时4.3 kDa、5.7 kDa、5.8 kDa、9.95 kDa和9.98 kDa蛋白质的表达增加。相比之下，处理后30分钟时6.3 kDa和8.6 kDa蛋白质的表达下降，48小时时4.9 kDa、5.0 kDa、12.4 kDa和19.8 kDa蛋白质的表达下降。11.3 kDa蛋白质被鉴定为具有多种功能的巨噬细胞迁移抑制因子（MIF）。9.98 kDa蛋白质被鉴定为与钙通道相关的钙结合蛋白。它们的表达时间表明MIF和钙结合蛋白参与雄激素对支持细胞生精后期的调节。
MIF和钙结合蛋白是支持细胞产生的两种重要的雄激素反应性蛋白，它们可能在调节生精过程中发挥作用。
