Sun Si Guo, Ma Bao An, Zhou Yong, Zhang Ming Hua, Fan Qing Yu
Department of Orthopedic Surgery, Tangdu Hospital, the Fourth Military Medical University, Xi'an, 710038. China.
Acta Orthop. 2006 Apr;77(2):320-8. doi: 10.1080/17453670610046091.
Despite the wide clinical use of bone cement, little is known about cellular responses to the debris from this material. We thus investigated the effects of bone cement particles on the secretion of soluble osteotropic factors in prosthetic pseudomembrane-derived fibroblasts.
Bone cement particles were added to fibroblasts maintained in tissue culture. The secretions of soluble receptor activator for nuclear factor kappa B ligand and osteoprotegerin together with interleukin-6 and tumor necrosis factor-alpha were assessed by enzyme-linked immunosorbent assays. The fibroblasts were also co-cultured with osteoclast precursors in the presence and absence of particles, and we assessed osteoclast formation and bone resorption.
The particles produced an increase in the secretion of soluble receptor activator for nuclear factor kappa B ligand, interleukin-6 and tumor necrosis factor-alpha, but not osteoprotegerin. At a concentration of 88 particles/cell, bone cement particles yielded a 2-fold increase (327 pg/mL) in soluble receptor activator for nuclear factor kappa B ligand secretion, a 5-fold (239 pg/mL) increase in interleukin-6 secretion and 4-fold (129 pg/mL) increase in tumor necrosis factor-alpha secretion. The particles also enhanced bone resorption in the co-culture group. Both the increase in soluble receptor activator for nuclear factor kappa B ligand secretion and the increase in bone resorption were inhibited by the addition of neutralizing antibodies to the proinflammatory cytokines.
Our findings show that bone cement particles are capable of stimulating the secretion of soluble receptor activator for nuclear factor kappa B ligand in pseudocapsule-derived fibroblasts by increasing the secretion of proinflammatory cytokines, and may also promote implant loosening.
尽管骨水泥在临床上广泛应用，但对于细胞对该材料碎片的反应却知之甚少。因此，我们研究了骨水泥颗粒对假体假膜来源的成纤维细胞中可溶性促骨因子分泌的影响。
将骨水泥颗粒添加到组织培养中的成纤维细胞中。通过酶联免疫吸附测定法评估核因子κB受体活化因子配体和骨保护素以及白细胞介素-6和肿瘤坏死因子-α的可溶性分泌。在有和没有颗粒存在的情况下，还将成纤维细胞与破骨细胞前体共培养，并评估破骨细胞的形成和骨吸收情况。
颗粒导致核因子κB受体活化因子配体、白细胞介素-6和肿瘤坏死因子-α的可溶性分泌增加，但骨保护素没有增加。在浓度为88个颗粒/细胞时，骨水泥颗粒使核因子κB受体活化因子配体分泌增加2倍（327 pg/mL），白细胞介素-6分泌增加5倍（239 pg/mL），肿瘤坏死因子-α分泌增加4倍（129 pg/mL）。颗粒还增强了共培养组中的骨吸收。添加针对促炎细胞因子的中和抗体可抑制核因子κB受体活化因子配体分泌的增加和骨吸收的增加。
我们的研究结果表明，骨水泥颗粒能够通过增加促炎细胞因子的分泌来刺激假包膜来源的成纤维细胞中核因子κB受体活化因子配体的可溶性分泌，并且还可能促进植入物松动。
