Ledniczky G, Fiore N, Bognár G, Ondrejka P, Grosfeld J L
2nd Department of Surgery, Semmelweis University Medical School Budapest, Hungary.
Chirurgia (Bucur). 2006 Mar-Apr;101(2):127-33.
Interleukin 11 (IL-11) is a multifunctional cytokine derived from bone marrow, which has a trophic effect on small bowel epithelium. This study compares the effects of IL-11 with epidermal growth factor (EGF), a growth factor known to enhance small bowel adaptation. Forty Sprague-Dawley rats (90-100g) underwent an 85% mid-small bowel resection with primary anastomosis on day 0. Rats were divided into four treatment groups: controls (group I) received bovine serum albumin (BSA), group II received IL-11, 125 microg/kg subcutaneously (SC) twice daily, group III received EGF, 0,10 microg/g SC bid, and group IV received EGF and IL-11 in the above doses. Half of the animals (five per group) were killed on day 4 of therapy, and the rest on day 8. Animals were evaluated for weight, mucosal length, and bowel wall muscle thickness on days 4 and 8, and expression of proliferating cell nuclear antigen (PCNA) in intestinal crypt and smooth muscle cells on day 8. Body weight was similar at day 4 and 8. Mucosal thickness in groups 11 (IL-11) and IV (IL-11 and EGF) was significantly increased at day 4 and 8 compared with controls (group I) and EGF (group III, P<.001). Muscle thickness was significantly increased in the EGF and combined group IV compared with the BSA controls and IL-11 groups (P < .001). Thirty-two percent of the mucosal crypt cells in group I stained positive for PCNA, whereas 51%, 53%, and 60% stained positive in groups II (IL-11), III (EGF), and IV (IL-11 and EGF), respectively. In groups I and II, 2% and 1.7% of the myocytes stained positive for PCNA, whereas 11.2% and 5.2% in group III and IV. These data suggest that IL-11 has a trophic effect on small intestinal enterocytes, causing cell proliferation and increased mucosal thickness. EGF has a more generalized effect causing proliferation of both enterocytes and myocytes. IL-11, with or without EGF may be a useful adjunct in treatment of short bowel syndrome.
白细胞介素11（IL-11）是一种源自骨髓的多功能细胞因子，对小肠上皮具有营养作用。本研究比较了IL-11与表皮生长因子（EGF）的作用，EGF是一种已知可增强小肠适应性的生长因子。40只斯普拉格-道利大鼠（90-100克）在第0天接受了85%的小肠中段切除并进行一期吻合术。大鼠被分为四个治疗组：对照组（I组）接受牛血清白蛋白（BSA）；II组每天两次皮下注射（SC）125微克/千克的IL-11；III组每天两次皮下注射0.10微克/克的EGF；IV组接受上述剂量的EGF和IL-11。治疗第4天处死一半动物（每组5只），其余在第8天处死。在第4天和第8天评估动物的体重、黏膜长度和肠壁肌肉厚度，并在第8天评估肠道隐窝和平滑肌细胞中增殖细胞核抗原（PCNA）的表达。第4天和第8天的体重相似。与对照组（I组）和EGF组（III组，P<0.001）相比，II组（IL-11）和IV组（IL-11和EGF）在第4天和第8天的黏膜厚度显著增加。与BSA对照组和IL-11组相比，EGF组和联合IV组的肌肉厚度显著增加（P<0.001）。I组32%的黏膜隐窝细胞PCNA染色呈阳性，而II组（IL-11）、III组（EGF）和IV组（IL-11和EGF）分别为51%、53%和60%。在I组和II组中，分别有2%和l.7%的肌细胞PCNA染色呈阳性，而III组和IV组分别为11.2%和5.2%。这些数据表明，IL-11对小肠肠上皮细胞具有营养作用，可导致细胞增殖和黏膜厚度增加。EGF具有更广泛的作用，可导致肠上皮细胞和平滑肌细胞均增殖。IL-11无论有无EGF，都可能是治疗短肠综合征的有用辅助药物。
