Salzmann J, Linderholm O P, Guyomard J-L, Paques M, Simonutti M, Lecchi M, Sommerhalder J, Dubus E, Pelizzone M, Bertrand D, Sahel J, Renaud P, Safran A B, Picaud S
Service d'Opthalmologie Hôpitaux Universitaires de Genève, Switzerland.
Br J Ophthalmol. 2006 Sep;90(9):1183-7. doi: 10.1136/bjo.2005.089110. Epub 2006 Jun 5.
In age related macular degeneration and inherited dystrophies, preservation of retinal ganglion cells has been demonstrated. This finding has led to the development of various models of subretinal or epiretinal implant in order to restore vision. This study addresses the development of a polyimide subretinal electrode platform in the dystrophic P23H rat in vivo.
A technique was developed for implanting a subretinal electrode into the subretinal space and stabilising the distal extremity of the cabling on the rat cranium in order to allow future electrical stimulations of the retina.
In vivo imaging of the retina with the scanning laser ophthalmoscope demonstrated reabsorption of the surgically induced retinal detachment and the absence of major tissue reactions. These in vivo observations were confirmed by retinal histology. The extraocular fixation system on the rat cranium was effective in stabilising the distal connector for in vivo stimulation.
This study demonstrates that a retinal implant can be introduced into the subretinal space of a dystrophic rat with a stable external connection for repeatable electrical measurements and stimulation. This in vivo model should therefore allow us to evaluate the safety and efficacy of electrical stimulations on dystrophic retina.
在年龄相关性黄斑变性和遗传性营养不良中，已证实视网膜神经节细胞得以保留。这一发现促使人们开发各种视网膜下或视网膜上植入模型以恢复视力。本研究探讨了在营养不良的P23H大鼠体内开发聚酰亚胺视网膜下电极平台的情况。
开发了一种将视网膜下电极植入视网膜下间隙并将电缆远端固定在大鼠颅骨上的技术，以便将来对视网膜进行电刺激。
用扫描激光检眼镜对视网膜进行体内成像显示，手术引起的视网膜脱离得到了吸收，且未出现重大组织反应。这些体内观察结果得到了视网膜组织学的证实。大鼠颅骨上的眼外固定系统有效地稳定了用于体内刺激的远端连接器。
本研究表明，可以将视网膜植入物引入营养不良大鼠的视网膜下间隙，并具有稳定的外部连接，用于可重复的电测量和刺激。因此，这种体内模型应能使我们评估对营养不良视网膜进行电刺激的安全性和有效性。
