Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo, Japan.
Biol Pharm Bull. 2006 Jun;29(6):1300-3. doi: 10.1248/bpb.29.1300.
Collagen-induced arthritis (CIA) is an experimental model of rheumatoid arthritis (RA) used for studying the clinical, immunological and genetic factors of the disease. Many studies of genetic susceptibility to CIA have been performed, usually with two strains of mice, DBA/1 and B10.RIII, since they are highly susceptible to CIA. Furthermore, F1 hybrid mice of susceptible and resistant strains reportedly develop arthritis. Recently, we reported that particles of beta-glucan, OX-CA, prepared from Candida albicans by NaClO-oxidation, acted as an adjuvant for CIA. Although, there have been many studies about the relationship between antigen and the major histocompatibility complex (MHC) in F1 hybrid mice, little is known argument about susceptibility to adjuvants. Therefore, we checked the susceptibility of F1 hybrids to CIA using OX-CA as an adjuvant. BALB/c and DBA/1 were mated to generate F1 hybrids which were then immunized with type II collagen (CII) plus Freund's complete adjuvant (FCA) or OX-CA. The results showed that F1 hybrids injected with CII plus FCA developed severe arthritis at an incidence ratio 80%, versus only 20% in mice injected with CII plus OX-CA. Furthermore, levels of anti-CII antibody, especially of the IgG2a subclass, in sera from mice treated with CII plus OX-CA were significantly low. Susceptibility to CIA might depend on not only MHC but also the adjuvant used for immunoactivation.
胶原诱导性关节炎（CIA）是一种类风湿关节炎（RA）的实验模型，用于研究疾病的临床、免疫和遗传因素。已经进行了许多关于 CIA 遗传易感性的研究，通常使用两种品系的小鼠，即 DBA/1 和 B10.RIII，因为它们对 CIA 高度易感。此外，据报道，易感和抗性品系的 F1 杂交小鼠也会发生关节炎。最近，我们报道了来自白色念珠菌的β-葡聚糖颗粒，OX-CA，通过 NaClO 氧化制备，可作为 CIA 的佐剂。尽管已经有许多关于 F1 杂交小鼠中抗原与主要组织相容性复合物（MHC）之间关系的研究，但关于佐剂易感性的研究却知之甚少。因此，我们使用 OX-CA 作为佐剂检查了 F1 杂种对 CIA 的易感性。将 BALB/c 和 DBA/1 交配以产生 F1 杂种，然后用 II 型胶原（CII）加福氏完全佐剂（FCA）或 OX-CA 免疫。结果表明，用 CII 加 FCA 注射的 F1 杂种关节炎发生率为 80%，而用 CII 加 OX-CA 注射的小鼠仅为 20%。此外，用 CII 加 OX-CA 处理的小鼠血清中抗 CII 抗体，特别是 IgG2a 亚类的水平明显较低。CIA 的易感性可能不仅取决于 MHC，还取决于用于免疫激活的佐剂。
