Fernandes M I, Zucoloto S, Collares E F, Ferriolli Filho F
Department of Puericulture and Pediatrics, Faculty of Medicine, University of São Paulo, Ribeirão Preto, Brasil.
Virchows Arch B Cell Pathol Incl Mol Pathol. 1991;60(2):119-22. doi: 10.1007/BF02899536.
The objective of this investigation was to study the morphometry of the epithelial mucosa in the chronic phase of T. cruzi infection. Nine young female Wistar rats were inoculated with T. cruzi. Ten months after inoculation the animals were sacrificed and the proximal colon was collected for morphometric measurements of the thickness of the muscle layers, the number of neurons in the myenteric plexus, the crypt cell population (CCP), crypt cell production per crypt (CCPC) and turnover time (TT) of the epithelium. There was no muscle layer hypertrophy but there was significant denervation in the group inoculated with T. cruzi, which also showed hyperplasia of the epithelium. The data suggest that denervation of the myenteric plexus did not induce hypertrophy of the propria muscle layer itself but altered the morphometry of the colonic epithelium in T. cruzi-infected animals, with increased development of CCP and TT. It is possible that this epithelial hyperplasia, as a consequence of a longer crypt cell TT, increased the absorption and secretion activities of the colon, which in turn may participate in the genesis of the enteromegalies observed in the chronic phase of Chagas' Disease.
本研究的目的是研究克氏锥虫感染慢性期上皮黏膜的形态学。9只年轻雌性Wistar大鼠接种克氏锥虫。接种10个月后处死动物，收集近端结肠，用于测量肌层厚度、肌间神经丛中神经元数量、隐窝细胞群（CCP）、每个隐窝的隐窝细胞产生量（CCPC）以及上皮细胞更新时间（TT）。接种克氏锥虫的组未出现肌层肥大，但存在明显的去神经支配，该组还表现出上皮增生。数据表明，肌间神经丛的去神经支配并未诱导固有肌层自身肥大，但改变了克氏锥虫感染动物结肠上皮的形态学，CCP和TT的发育增加。由于隐窝细胞TT延长导致的这种上皮增生，可能增加了结肠的吸收和分泌活动，这反过来可能参与恰加斯病慢性期观察到的巨结肠的发生。
