Pereira Gislaine S P, Brandão Rodrigo M, Giuliatti Silvana, Zago Marco A, Silva Wilson A
Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, USP, Ribeirão Preto, SP, Brazil.
Genet Mol Res. 2006 Mar 31;5(1):108-14.
Serial analysis of gene expression (SAGE) technology produces large sets of interesting genes that are difficult to analyze directly. Bioinformatics tools are needed to interpret the functional information in these gene sets. We present an interactive web-based tool, called Gene Class, which allows functional annotation of SAGE data using the Gene Ontology (GO) database. This tool performs searches in the GO database for each SAGE tag, making associations in the selected GO category for a level selected in the hierarchy. This system provides user-friendly data navigation and visualization for mapping SAGE data onto the gene ontology structure. This tool also provides graphical visualization of the percentage of SAGE tags in each GO category, along with confidence intervals and hypothesis testing.
基因表达序列分析（SAGE）技术产生了大量难以直接分析的有趣基因集。需要生物信息学工具来解读这些基因集中的功能信息。我们展示了一种基于网络的交互式工具，名为基因分类器（Gene Class），它允许使用基因本体论（GO）数据库对SAGE数据进行功能注释。该工具针对每个SAGE标签在GO数据库中进行搜索，在层次结构中所选级别对应的选定GO类别中建立关联。该系统为将SAGE数据映射到基因本体结构提供了用户友好的数据导航和可视化功能。此工具还提供每个GO类别中SAGE标签百分比的图形可视化，以及置信区间和假设检验。
