[精液质量与精子顶体酶活性关系的分析]

[Analysis of relationship between semen quality and sperm acrosin activity].

作者信息

Xu Shu-Ping, Zhan Bao-E

机构信息

Department of Clinical Laboratory, Shenzhen Hospital of Traditional Chinese Medicine, Shenzhen, Guangdong 518033, China.

出版信息

Zhonghua Nan Ke Xue. 2006 May;12(5):438-40.

PMID:16755877

Abstract

OBJECTIVE

To analyze the activity of human sperm acrosin and semen parameters in male infertile patients and discuss the effect of sperm acrosin activity on semen quality.

METHODS

Activity of human sperm acrosin, PMN-elastase, fructose, alpha-glucosidase, zinc, acid phosphatase levels and HOST of 214 male infertile patients were detected. Semen analysis was performed using WLJY-9000 WeiLi colorful semen quality analyzer. There were 111 cases of normal activity of human sperm acrosin (48.2 - 218.7 microIU/10(6) sperm), 103 cases abnormal (< 48.2 microIU/10(6) sperm). Using the group of normal activity of sperm acrosin to be the control, semen parameters was analyzed and compared with those of the group of abnormal activity of sperm acrosin.

RESULTS

There were significant difference (P < 0.001) between the 2 groups (normal and abnormal) in the areas of sperm density, motile sperm rate, percentage of grade (a + b) sperm and HOST. There were also significant difference in PMN-elastase, fructose and alpha-glucosidase (P < 0.05). There was no difference among sperm volume, zinc and acid phosphatase (P > 0.05).

CONCLUSION

There was a strong correlation between the activity of human sperm sperm acrosin and semen quality. Activity of sperm acrosin is a reliable index of semen quality.

摘要

目的

分析男性不育患者的人类精子顶体酶活性及精液参数，探讨精子顶体酶活性对精液质量的影响。

方法

检测214例男性不育患者的人类精子顶体酶活性、中性粒细胞弹性蛋白酶、果糖、α-葡萄糖苷酶、锌、酸性磷酸酶水平及宿主抗精子抗体（HOST）。使用WLJY-9000伟力彩色精液质量分析仪进行精液分析。人类精子顶体酶活性正常（48.2 - 218.7微国际单位/10⁶精子）的有111例，异常（<48.2微国际单位/10⁶精子）的有103例。以精子顶体酶活性正常组为对照，分析并比较精子顶体酶活性异常组的精液参数。