Rekstin A R, Lu K, Kats D, Rudenko L G
Vopr Virusol. 2006 Mar-Apr;51(2):27-30.
The production of proinflammatory cytokines was studied following experimental infection of BALB/c mice with influenza viruses that differed in virulence. The generation of TNF-alpha, IL-6, IL-12, and IFN-gamma was investigated in the lung homogenates in the early periods after intranasal infection of mice with A/Leningrad/134/57 (H2N2) wild-type virus and cold-adapted attenuated vaccine viruses: A/Leningrad/134/17157 (H2N2) and A/Leningrad/134/47/57 (H2N2). Wild-type virus induced substantially higher levels of proinflammatory cytokines: TNF-alpha, IL-6, IL-12, and IFN-gamma. After infection with the cold-adapted viruses, the levels of the cytokines were reduced as compared to those induced by the wild-type virus. The A/Leningrad/134/47/57 virus was marked by a noticeable production of IL-6 and IFN-gamma in the murine lung, but it was less than with wild-type virus infection. At the same time, the more attenuated strain A/Leningrad/134/47/57 induced TNF-alpha and IFN-gamma in the quantities similar to those in the control animals. Thus, a response of proinflammatory cytokines in early infection in the murine lung depended on the level of viral replication in the lower respiratory tract and on the attenuation of influenza virus strains.
在用毒力不同的流感病毒对BALB/c小鼠进行实验性感染后，研究了促炎细胞因子的产生情况。在用A/列宁格勒/134/57（H2N2）野生型病毒以及冷适应减毒疫苗病毒：A/列宁格勒/134/17157（H2N2）和A/列宁格勒/134/47/57（H2N2）经鼻感染小鼠后的早期，对肺匀浆中肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）、白细胞介素-12（IL-12）和干扰素-γ（IFN-γ）的生成情况进行了研究。野生型病毒诱导产生的促炎细胞因子水平显著更高：TNF-α、IL-6、IL-12和IFN-γ。与野生型病毒诱导的水平相比，在用冷适应病毒感染后，细胞因子水平降低。A/列宁格勒/134/47/57病毒的特点是在小鼠肺中显著产生IL-6和IFN-γ，但低于野生型病毒感染时的水平。同时，减毒程度更高的A/列宁格勒/134/47/57毒株诱导产生的TNF-α和IFN-γ数量与对照动物相似。因此，小鼠肺早期感染中促炎细胞因子的反应取决于下呼吸道中病毒复制的水平以及流感病毒毒株的减毒程度。
