Taniai K, Lee J H, Lee I H
Insect Biotechnology and Sericology Department, National Institute of Agrobiological Sciences, Ibaraki, Japan.
Insect Mol Biol. 2006 Jun;15(3):269-79. doi: 10.1111/j.1365-2583.2006.00639.x.
We tested 11 Bombyx mori cell lines for induction of cecropin B gene (CecB) expression. After the immune challenge, CecB expression was induced in seven cell lines. A mixture of the cell-free supernatant from the immune-responsive cell lines and lipopolysaccharide activated a promoter of CecB in the non-immune-responsive cell line, indicating that secreted factor(s) is involved in CecB activation. The expressed sequence tags of one of the immune-responsive cell lines, NISES-BoMo-Cam1, contained genes encoding proteins similar to Relish, Cactus, clip-domain serine protease, serpin, lectin, peptidoglycan recognition protein, 6tox and gloverin, in addition to seven known B. mori immune-inducible genes. These results show that NISES-BoMo-Cam1 cells can be used as an in vitro model of the immune system organs of B. mori.
我们检测了11种家蚕细胞系中杀菌肽B基因（CecB）表达的诱导情况。免疫刺激后，7种细胞系中诱导了CecB表达。来自免疫反应性细胞系的无细胞上清液与脂多糖的混合物激活了非免疫反应性细胞系中CecB的启动子，表明分泌因子参与了CecB的激活。免疫反应性细胞系之一NISES-BoMo-Cam1的表达序列标签，除了包含7个已知的家蚕免疫诱导基因外，还含有编码与Relish、Cactus、clip结构域丝氨酸蛋白酶、丝氨酸蛋白酶抑制剂、凝集素、肽聚糖识别蛋白、6tox和gloverin相似的蛋白质的基因。这些结果表明，NISES-BoMo-Cam1细胞可作为家蚕免疫系统器官的体外模型。
