Bonneton F, Brunet F G, Kathirithamby J, Laudet V
Laboratoire de Biologie Moléculaire de la Cellule, UMR 5161 du CNRS, INRA LA 1237, IFR128 BioSciences Lyon-Gerland, Ecole Normale Supérieure de Lyon, 46 Allée d'Italie, Lyon, France.
Insect Mol Biol. 2006 Jun;15(3):351-62. doi: 10.1111/j.1365-2583.2006.00654.x.
In arthropods, the regulation by ecdysteroids is mediated by the heterodimer between the ecdysone receptor (ECR; NR1H1) and ultraspiracle (USP/RXR; NR2B4) nuclear receptors. Both ECR and USP/RXR ligand-binding domains experienced a strong acceleration of evolutionary rate in Diptera and Lepidoptera, which belong to the superorder Mecopterida. We performed a phylogenetic analysis of 28 ECR and 30 USP/RXR protein sequences from 36 arthropod species, including representatives from Trichoptera, Mecoptera and Siphonaptera. Our data show that the acceleration of ECR and USP/RXR was a unique event in the ancestor of Mecopterida. Our analysis shows further that Strepsiptera ECR and USP/RXR sequences are unambiguously placed outside of the Mecopterida clade. Protein alignments reveal that eight of 11 synapomorphies support an affinity between Strepsiptera and Coleoptera sequences. The affiliation of Strepsiptera to Diptera should therefore be rejected.
在节肢动物中，蜕皮甾体的调控是由蜕皮激素受体（ECR；NR1H1）和超气门蛋白（USP/RXR；NR2B4）核受体之间的异二聚体介导的。ECR和USP/RXR的配体结合结构域在双翅目和鳞翅目（属于长翅总目）中经历了进化速率的强烈加速。我们对来自36种节肢动物的28个ECR和30个USP/RXR蛋白序列进行了系统发育分析，其中包括毛翅目、长翅目和蚤目的代表物种。我们的数据表明，ECR和USP/RXR的加速是长翅总目祖先中的一个独特事件。我们的分析进一步表明，捻翅目ECR和USP/RXR序列明确位于长翅总目分支之外。蛋白质比对显示，11个共有衍征中的8个支持捻翅目和鞘翅目序列之间的亲缘关系。因此，捻翅目与双翅目的亲缘关系应被否定。
