Meyer O, Abdallah Z, Schönemann C, Hugo F, Agaylan A, Kiesewetter H, Salama A
Charité, Universitätsmedizin Berlin, Institut für Transfusionsmedizin, Berlin, Germany.
Vox Sang. 2006 Jul;91(1):77-80. doi: 10.1111/j.1423-0410.2006.00786.x.
The human leucocyte antigen (HLA) B27 is the most frequently typed single antigen that is associated with diseases. Here, we describe a simple and rapid particle agglutination assay (PaGIA) for HLA-B27 typing.
Superparamagnetic particles were coated with a monoclonal antibody to HLA-B27 and subsequently used for testing. Anticoagulated whole-blood samples were obtained from healthy blood donors (n = 194) with known HLA patterns and from patients (n = 51) who had been typed positive for HLA-B27 by flow cytometry.
The particles agglutinated only after incubation with HLA-27-positive blood samples, using the ID-microtyping system. Positive reactions were clearly distinguishable from negative reactions in all samples tested. Flow cytometric HLA-B27 typing revealed an indeterminate result in one patient.
The new HLA-B27 PaGIA is suitable for rapid typing of HLA-B27. The assay is simple and easy to perform, and can be implemented in any routine laboratory.
人类白细胞抗原（HLA）-B27是与疾病相关的最常检测的单一抗原。在此，我们描述一种用于HLA-B27分型的简单快速的颗粒凝集试验（PaGIA）。
用抗HLA-B27单克隆抗体包被超顺磁性颗粒，随后用于检测。从已知HLA分型的健康献血者（n = 194）和通过流式细胞术检测HLA-B27呈阳性的患者（n = 51）中获取抗凝全血样本。
使用ID微分型系统，颗粒仅在与HLA-27阳性血样孵育后发生凝集。在所有检测样本中，阳性反应与阴性反应明显可区分。流式细胞术HLA-B27分型显示一名患者结果不确定。
新的HLA-B27 PaGIA适用于HLA-B27的快速分型。该检测方法简单易操作，可在任何常规实验室开展。
Zotero 插件