通过9-均三甲苯基-10-甲基吖啶离子的电子转移状态对DNA碱基进行电子转移氧化产生核苷酸自由基阳离子的直接检测以及无氧条件下高效的DNA切割。

Direct detection of nucleotide radical cations produced by electron-transfer oxidation of DNA bases with electron-transfer state of 9-mesityl-10-methylacridinium ion and resulting efficient DNA cleavage without oxygen.

作者信息

Ohkubo Kei, Yukimoto Kazusa, Fukuzumi Shunichi

机构信息

Department of Material and Life Science, Graduate School of Engineering, Osaka University, SORST, Japan Science and Technology Agency (JST), Suita, Osaka, 565-0871, Japan.

出版信息

Chem Commun (Camb). 2006 Jun 21(23):2504-6. doi: 10.1039/b601418f. Epub 2006 May 8.

DOI:10.1039/b601418f

PMID:16758030

Abstract

Photoinduced electron transfer of DNA as well as DNA bases with 9-mesityl-10-methylacridinium ion results in formation of all types of DNA base radical cations, which have been detected as the transient absorption spectra measurements, leading to efficient DNA cleavage in the absence of O2.

摘要

DNA以及DNA碱基与9-均三甲苯基-10-甲基吖啶离子的光致电子转移导致形成所有类型的DNA碱基自由基阳离子，这些阳离子已通过瞬态吸收光谱测量检测到，从而在无氧条件下实现高效的DNA切割。