Baharvand Hossein, Hajheidari Mohsen, Ashtiani Saeid Kazemi, Salekdeh Ghasem Hosseini
Department of Stem Cells, Royan Institute, Tehran, Iran.
Proteomics. 2006 Jun;6(12):3544-9. doi: 10.1002/pmic.200500844.
Human embryonic stem cells (hESC) represent a population of undifferentiated pluripotent cells with both self-renewal and multilineage differentiation characteristics. Proteomics provides a powerful approach for studying the characteristics of hESC and discovering molecular markers. We have analyzed proteome profiles of three hESC lines using 2-DE and MALDI TOF-TOF. Out of 844 spots analyzed with MALDI TOF-TOF, 685 proteins were identified of which 60 proteins were classified as the most abundant proteins on 2-D gels. A large number of proteins particularly high abundant ones were identified as chaperones, heat shock proteins, ubiquitin/proteasome, and oxidative stress responsive proteins underscoring the ability of these cells to resist oxidative stress and increase the life span. Several proteins involved in cell proliferation and differentiation were also among the highly expressed proteins. Although overall expression pattern of three hESC were similar, 54 spots changed quantitatively and 14 spots changed qualitatively among the hESC cell lines. Most of these proteins were identified as proteins involved in cell growth, metabolism and signal transduction, which may affect the self-renewal and pluripotency. To our knowledge, this study represents the first proteomic dataset for hESC and provides a better insight into the biology of hESC. Proteome maps of hESC are accessible at http://www.RoyanProteomics.ir.
人类胚胎干细胞（hESC）代表了一群具有自我更新和多向分化特征的未分化多能细胞。蛋白质组学为研究hESC的特征和发现分子标志物提供了一种强大的方法。我们使用二维电泳（2-DE）和基质辅助激光解吸电离飞行时间串联质谱（MALDI TOF-TOF）分析了三种hESC系的蛋白质组图谱。在用MALDI TOF-TOF分析的844个斑点中，鉴定出685种蛋白质，其中60种蛋白质被归类为二维凝胶上最丰富的蛋白质。大量蛋白质，特别是高丰度蛋白质，被鉴定为伴侣蛋白、热休克蛋白、泛素/蛋白酶体和氧化应激反应蛋白，突出了这些细胞抵抗氧化应激和延长寿命的能力。一些参与细胞增殖和分化的蛋白质也在高表达蛋白质之中。尽管三种hESC的总体表达模式相似，但在hESC细胞系中，有54个斑点在数量上发生了变化，14个斑点在质量上发生了变化。这些蛋白质大多被鉴定为参与细胞生长、代谢和信号转导的蛋白质，这可能会影响自我更新和多能性。据我们所知，这项研究代表了hESC的第一个蛋白质组数据集，并为深入了解hESC的生物学特性提供了更好的视角。hESC的蛋白质组图谱可在http://www.RoyanProteomics.ir上获取。
