钙激活中性蛋白酶（CANP）在周围神经中的定位。

Localization of calcium-activated neutral protease (CANP) in the peripheral nerve.

作者信息

Kamakura K, Ishiura S, Fujita T, Nonaka I, Sugita H

机构信息

Division of Neuromuscular Research, National Center for Nervous, Mental and Muscular Disorders, Kodaira, Tokyo, Japan.

出版信息

Muscle Nerve. 1985 Jun;8(5):380-2. doi: 10.1002/mus.880080506.

DOI:10.1002/mus.880080506

PMID:16758583

Abstract

Localization of calcium-activated neutral protease (CANP) in the rat peripheral nerve was studied by an indirect immunofluorescent method, using rabbit antiserum against CANP extracted from chicken skeletal muscle. Its specificity to CANP, as well as its cross-reactivity with rat CANP, were confirmed by the Ouchterlony immunodiffusion procedure and immunoreplica method. CANP was demonstrated in the axon, but not in the myelin sheath or endoneurium. We could not conclude whether or not CANP in axons exists in association with neurofilaments.

摘要

采用间接免疫荧光法，使用针对从鸡骨骼肌中提取的钙激活中性蛋白酶（CANP）的兔抗血清，研究了大鼠周围神经中CANP的定位。通过双向免疫扩散法和免疫印迹法证实了其对CANP的特异性以及与大鼠CANP的交叉反应性。CANP在轴突中被检测到，但在髓鞘或神经内膜中未被检测到。我们无法确定轴突中的CANP是否与神经丝相关联。