兔和鸡骨骼肌中钙激活中性蛋白酶的比较。

Comparison of calcium-activated neutral proteases from skeletal muscle of rabbit and chicken.

作者信息

Kawashima S, Nomoto M, Hayashi M, Inomata M, Nakamura M, Imahori K

出版信息

J Biochem. 1984 Jan;95(1):95-101. doi: 10.1093/oxfordjournals.jbchem.a134608.

DOI:10.1093/oxfordjournals.jbchem.a134608

Abstract

Calcium-activated neutral proteases (CANPs) were purified from rabbit skeletal muscle and chicken skeletal muscle, and compared as to their electrophoretic properties, metal requirements, subunit amino acid compositions and immunological cross-reactivities. Two kinds of CANPs (mu CANP and mCANP) were isolated from rabbit but the chicken tissue lacked one corresponding to mu CANP. They were acidic in the order of chicken mCANP, rabbit mCANP, and rabbit mu CANP but the difference between the former two was very small. All of them were composed of two subunits, so-called 80K and 30K subunits. The molecular weight of the 30K subunit was the same for these CANPs (28K) but those of the 80K subunit were different (79K for rabbit mu CANP, 75K for rabbit mCANP and 81K for chicken mCANP). The calcium-sensitivity of chicken mCANP was very high when compared with that of rabbit mCANP and close to that of rabbit mu CANP. Antisera against chicken CANP and those against rabbit CANP cross-reacted with rabbit CANP and chicken CANP, respectively, when examined by immunoelectrotransfer blot techniques.

摘要

从兔骨骼肌和鸡骨骼肌中纯化出钙激活中性蛋白酶（CANPs），并对它们的电泳性质、金属需求、亚基氨基酸组成和免疫交叉反应性进行了比较。从兔中分离出两种CANPs（μCANP和mCANP），但鸡组织中缺乏与μCANP相对应的一种。它们的酸性顺序为鸡mCANP、兔mCANP和兔μCANP，但前两者之间的差异非常小。它们均由两个亚基组成，即所谓的80K和30K亚基。这些CANPs的30K亚基分子量相同（28K），但80K亚基的分子量不同（兔μCANP为79K，兔mCANP为75K，鸡mCANP为81K）。与兔mCANP相比，鸡mCANP对钙的敏感性非常高，且接近兔μCANP。当通过免疫电转移印迹技术检测时，抗鸡CANP的抗血清和抗兔CANP的抗血清分别与兔CANP和鸡CANP发生交叉反应。