Nifontova I N, Drize N I
Hematology Research Center, Russian Academy of Medical Sciences, Moscow.
Bull Exp Biol Med. 2005 Nov;140(5):550-3. doi: 10.1007/s10517-006-0021-7.
The effect of TNF expression by wild type stromal sublayer on hemopoiesis was studied in cultures after second inoculation of TNF-deficient bone marrow cells. Long-term maintenance of hemopoiesis was determined solely by the absence of autocrine expression of TNF by hemopoietic cells. The production of hemopoietic precursors of all studied types in TNF-deficient cultures on a wild type sublayer was significantly higher than in cultures without TNF production. Presumably, initial expression of TNF in the sublayer promotes the survival of hemopoietic precursors with high proliferative potential in the culture. It is also obvious that TNF is required for normal functioning of stromal precursor cells.
在第二次接种肿瘤坏死因子（TNF）缺陷型骨髓细胞后的培养中，研究了野生型基质亚层表达的TNF对造血的影响。造血的长期维持完全取决于造血细胞自分泌TNF的缺失。在野生型亚层的TNF缺陷型培养物中，所有研究类型的造血前体的产生明显高于无TNF产生的培养物。据推测，亚层中TNF的初始表达促进了培养物中具有高增殖潜力的造血前体的存活。同样明显的是，TNF是基质前体细胞正常功能所必需的。
