[溴氰菊酯染毒大鼠脑组织中γ-谷氨酰半胱氨酸合成酶亚基及Nrf2基因表达的时间进程]

[Time course of gene expression of gamma-glutamylcysteine synthetase subunit and Nrf2 in brain tissues of rats exposed by deltamethrin].

作者信息

Li Huang-yuan, Shi Nian, Zhong Yu-fang, Dai Zhong-hua

机构信息

Department of Health Toxicology, MOE Key Lab of Enviornment and health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

出版信息

Wei Sheng Yan Jiu. 2006 Mar;35(2):130-4.

PMID:16758951

Abstract

OBJECTIVE

To investigate the time course of mRNA expression of gamma- glutamylcysteine synthetase light (GCSl) and heavy subunit (GCSh), as well as protein expression of both GCSh and NF-E2 related factor 2 (Nrf2) in cerebral cortex and hippocampus in rats exposed at single dose of deltamethrin (DM).

METHODS

Wistar male rats were exposed at single dose of DM (12.50 mg/kg bw) with i.p. At various time points of post-exposure, the rats were sacrificed at indicated time, then their cerebral cortex and hippocampus were isolated. The relative amount of mRNA expression of these genes was measured by the method of reverse transcription polymerase chain reaction (RT-PCR). The protein level was detected by the method of immunohistochemistry and image analysis system.

RESULTS

(1) At 5 and 48h after DM exposure, GCSh mRNA relative levels in cerebral cortex in rats were significantly decreased to 83.9% and 86.0% of mRNA level of corresponding tissue of control group at Oh, respectively (P < 0.05). At 5, 24 and 48h after DM exposure, transcriptional factors Nrf2 mRNA relative levels were more higher than those of both control and 72 h group and increased to 146.4%, 145.2% and 147.9% of those of control group at Oh,respectively (P < 0.05). (2) At 5 and 48h after DM exposure, GCSh mRNA relative levels in hippocampus were significantly increased to 118.4% and 118.4% of those of control group at 0h, respectively (P < 0.05). At 5h after DM exposure, GCSl mRNA relative levels in hippocampus were more higher than those of control,24h and 48h group and increased to 121.4% of those of control group at Oh (P < 0.05).

CONCLUSION

After single dose of DM exposure, there are up-regulation of mRNA expression of both GCSh and GCSl gene in rats hippocampus, down-regulation of mRNA expression of GCSh gene and up-regulation of mRNA expression of Nrf2. gene in rats cerebral cortex under the experimental condition.

摘要

目的

研究单次给予溴氰菊酯（DM）后，大鼠大脑皮层和海马中γ-谷氨酰半胱氨酸合成酶轻亚基（GCSl）和重亚基（GCSh）的mRNA表达时程，以及GCSh和核因子E2相关因子2（Nrf2）的蛋白表达情况。

方法

将Wistar雄性大鼠单次腹腔注射给予DM（12.50mg/kg体重）。在暴露后的不同时间点，于指定时间处死大鼠，然后分离其大脑皮层和海马。采用逆转录聚合酶链反应（RT-PCR）法检测这些基因的mRNA相对表达量。通过免疫组织化学和图像分析系统检测蛋白水平。

结果

（1）DM暴露后5小时和48小时，大鼠大脑皮层中GCSh mRNA相对水平分别显著降至0小时对照组相应组织mRNA水平的83.9%和86.0%（P<0.05）。DM暴露后5小时、24小时和48小时，转录因子Nrf2 mRNA相对水平均高于对照组和72小时组，分别升至0小时对照组的146.4%、145.2%和147.9%（P<0.05）。（2）DM暴露后5小时和48小时，海马中GCSh mRNA相对水平分别显著升至0小时对照组的118.4%和118.4%（P<0.05）。DM暴露后5小时，海马中GCSl mRNA相对水平高于对照组、24小时组和48小时组，升至0小时对照组的121.4%（P<0.05）。