[Determination of the concentration of ropivacaine in dog plasma by reversed-phase high performance liquid chromatography].

OBJECTIVE

To establish a Reversed-Phase high performance liquid chromatography method for determination of plasma ropivacaine in dog.

METHODS

The concentration of ropivacaine was assayed on Intertsil C18 column (4.6 mm x 250 mm, 5 microm)with a mobile phase consisting of methanol: H2O (50:50), the detection wavelength was 215nm and the flow rate was 1.4 ml/min. the column temperature was 20 degrees C and the sensitivity was 0.02 AUFS respectively. the volume of sample for detection is 10 microl.

RESULTS

the linear range was 0.1-25 microg/ml (r = 0.9982). The recovery of ropivacaine was 91.2%-93.6%, RSD were 2.10%-3.40% (n=6). The detection limit was 0.05 microg/ml. the intra-day and interday precisions of assay for ropivacaine was 1.35%-2.88% and 1.80%-3.76%.

CONCLUSION

This method is simple, rapid, accurate and convenient for determination of the concentration of ropivacaine in dog plasma.