Wang Jia-xiang, Wang Li, Fan Ying-zhong, Liu Qiu-liang, Zhang Jiao, Yu Jie-kai, Zheng Shu
Department of Surgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China.
Zhonghua Yi Xue Za Zhi. 2006 Apr 11;86(14):979-82.
To detect new biomarkers and to establish a serum protein fingerprint model for early detection and diagnosis of thyroid cancer.
The serum samples of 40 thyroid cancer patients, 9 thyroid adenoma patient, and 32 healthy individuals were randomly divided into 2 sets: training set (n = 66, including 32 thyroid cancer patients, 9 thyroid adenoma patients, and 25 healthy individuals) and test set (n = 15). The serum protein were bound to WCX2 chip and tested by surface enhanced laser desorption/ionization time of flight-mass spectrometry (SELDI-TOF-MS). The data of spectra were analyzed by support vector machine (SVM) to establish a diagnostic model.
The detective model combined with 3 biomarkers could differentiate the serum of thyroid cancer from that of healthy individual with a specificity of 86% and a sensitivity of 100%. The diagnostic model combined with 3 biomarkers could differentiate thyroid cancer from thyroid adenoma with a specificity of 88.9% and a sensitivity of 96.9%. The positive predictive value to differentiate papillary thyroid carcinoma from the thyroid cancer of other types was 97%, and the positive predictive value of thyroid carcinoma of other pathological types was 71%.
The combination of SELDI with bioinformatics tools is a novel, effective, and highly specific and sensitive method for thyroid cancer detection and diagnosis.
