多种金属对人外周血淋巴细胞体外DNA合成及淋巴因子产生的影响。

Effects of various metals on DNA synthesis and lymphokines production by human peripheral blood lymphocytes in vitro.

Theocharis S, Margeli A, Panayiotidis P

First Department of Internal Medicine, School of Medicine, University of Athens, Laikon Hospital, Greece.

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1991;99(1-2):131-3. doi: 10.1016/0742-8413(91)90088-b.

The presence of Cd2+, Ni2+, Cu2+, Mn2+ and Co2+ (10(-4)-10(-6) M) caused a dose-dependent inhibition of DNA synthesis in phytohemagglutinin (PHA) stimulated peripheral blood mononuclear cells (PBMC) from normal human donors. No inhibition of DNA synthesis was observed in the presence of Mg2+ and Al3+. 2. Spontaneous and PHA induced interleukin-1 (IL-1) was enhanced in the presence of Mn2+. 3. The release of interleukin-2 (IL-2) and soluble interleukin-2 receptors (sIL-2R) was decreased by Cd2+, Cu2+ and Mn2+. 4. Spontaneous tumor necrosis factor-alpha (TNF-alpha) production was enhanced by Co2+ and Al3+, but decreased by Cd2+. 5. gamma-Interferon (gamma-IFN) production was decreased in the presence of Cd2+ and Cu2+ and increased in the presence of Al3+.

Cd2+、Ni2+、Cu2+、Mn2+和Co2+（10^(-4)-10^(-6) M）的存在会对来自正常人类供体的植物血凝素（PHA）刺激的外周血单个核细胞（PBMC）中的DNA合成产生剂量依赖性抑制。在Mg2+和Al3+存在的情况下未观察到DNA合成受到抑制。2. 在Mn2+存在的情况下，自发的和PHA诱导的白细胞介素-1（IL-1）会增加。3. Cd2+、Cu2+和Mn2+会降低白细胞介素-2（IL-2）和可溶性白细胞介素-2受体（sIL-2R）的释放。4. Co2+和Al3+会增强自发的肿瘤坏死因子-α（TNF-α）产生，但Cd2+会使其降低。5. 在Cd2+和Cu2+存在的情况下，γ-干扰素（γ-IFN）产生减少，而在Al3+存在的情况下增加。

Effects of various metals on DNA synthesis and lymphokines production by human peripheral blood lymphocytes in vitro.