Gresack Jodi E, Frick Karyn M
Department of Psychology, Yale University, P.O. Box 208205, New Haven, CT 06520, USA.
Pharmacol Biochem Behav. 2006 May;84(1):112-9. doi: 10.1016/j.pbb.2006.04.013. Epub 2006 Jun 6.
The present study was designed to determine if post-training injections of a water-soluble form of 17beta-estradiol could enhance spatial and object memory consolidation in young female mice. Young ovariectomized female mice were trained in Morris water maze and object recognition tasks, injected with 0.1, 0.2, or 0.4 mg/kg cyclodextrin-encapsulated 17beta-estradiol or cyclodextrin-conjugated vehicle, and then re-tested after a delay. In the water maze, mice were trained in eight consecutive trials, injected, and memory for the platform location was re-tested after 24 h. All mice learned to find the platform on Day 1, but only mice receiving 0.2 mg/kg estradiol remembered the platform location on Day 2. In the object recognition task, mice were first presented with two identical objects, injected, and then presented with a familiar and novel object after a 24- or 48-h delay. For both delays, the 0.2 and 0.4 mg/kg doses enhanced memory for the familiar object. These data demonstrate that a 0.2 mg/kg dose of estradiol can enhance multiple types of memory consolidation in female mice, and suggest a narrower effective dose range for spatial memory than for object memory.
本研究旨在确定训练后注射水溶性形式的17β-雌二醇是否能增强年轻雌性小鼠的空间和物体记忆巩固。将年轻的去卵巢雌性小鼠进行莫里斯水迷宫和物体识别任务训练，注射0.1、0.2或0.4mg/kg环糊精包封的17β-雌二醇或环糊精结合的载体，然后在延迟后重新测试。在水迷宫中，小鼠连续进行八次试验，注射后，在24小时后重新测试对平台位置的记忆。所有小鼠在第1天学会找到平台，但只有接受0.2mg/kg雌二醇的小鼠在第2天记住了平台位置。在物体识别任务中，小鼠首先被呈现两个相同的物体，注射后，在24或48小时延迟后再被呈现一个熟悉的和一个新的物体。对于这两种延迟，0.2和0.4mg/kg剂量增强了对熟悉物体的记忆。这些数据表明，0.2mg/kg剂量的雌二醇可以增强雌性小鼠多种类型的记忆巩固，并表明空间记忆的有效剂量范围比物体记忆更窄。
