Naghibalhossaini Fakhraddin, Moaddeb Afsaneh
Department of Biochemistry, Shiraz University of Medical Sciences, Zand Street, Shiraz 71345, Iran.
Biomol Eng. 2006 Sep;23(4):209-12. doi: 10.1016/j.bioeng.2006.04.002. Epub 2006 Apr 29.
The PCR primers used for cloning of evolutionary conserved genes or homologous DNA sequences are usually guessmer oligonucleotides. We introduce a simple way using Pfu polymerase to overcome possible PCR amplification failure because of 3'-end mismatches of guessed primers with the target DNA.
用于克隆进化保守基因或同源DNA序列的PCR引物通常是推测性寡核苷酸。我们介绍一种使用Pfu聚合酶的简单方法，以克服由于推测引物与目标DNA的3'端错配而可能导致的PCR扩增失败。
