一种改进的基于聚合酶链式反应的未知同源DNA序列扩增方法。

An improved PCR-based amplification of unknown homologous DNA sequences.

作者信息

Naghibalhossaini Fakhraddin, Moaddeb Afsaneh

机构信息

Department of Biochemistry, Shiraz University of Medical Sciences, Zand Street, Shiraz 71345, Iran.

出版信息

Biomol Eng. 2006 Sep;23(4):209-12. doi: 10.1016/j.bioeng.2006.04.002. Epub 2006 Apr 29.

DOI:10.1016/j.bioeng.2006.04.002

PMID:16759909

Abstract

The PCR primers used for cloning of evolutionary conserved genes or homologous DNA sequences are usually guessmer oligonucleotides. We introduce a simple way using Pfu polymerase to overcome possible PCR amplification failure because of 3'-end mismatches of guessed primers with the target DNA.

摘要

用于克隆进化保守基因或同源DNA序列的PCR引物通常是推测性寡核苷酸。我们介绍一种使用Pfu聚合酶的简单方法，以克服由于推测引物与目标DNA的3'端错配而可能导致的PCR扩增失败。