Kyama Cleophas M, Overbergh Lutgart, Debrock Sophie, Valckx Dirk, Vander Perre Sarah, Meuleman Christel, Mihalyi Attila, Mwenda Jason M, Mathieu Chantal, D'Hooghe Thomas M
Leuven University Fertility Centre, Department of Obstetrics and Gynaecology, University Hospital Gasthuisberg, Leuven, Belgium.
Fertil Steril. 2006 Jun;85(6):1667-75. doi: 10.1016/j.fertnstert.2005.11.060.
To examine differential messenger RNA (mRNA) expression of relevant cytokines, metalloproteases, growth and adhesion factors in endometrium and peritoneum from women with endometriosis when compared with women without the disease during menstrual and luteal phases of the cycle.
Patients with endometriosis were compared with control patients.
University hospital.
PATIENT(S): A total of 35 patients (20 patients during the luteal phase and 15 patients during the menstrual phase) were selected for this study on the basis of cycle phase and presence or absence of endometriosis.
INTERVENTION(S): In this study, endometriosis was laparoscopically and histologically confirmed in 24 women with endometriosis of revised American Society for Reproductive Medicine (ASRM) stage I-II (n = 12) and revised ASRM stage III-IV (n = 12), and the presence of a normal pelvis was documented by laparoscopy in 11 control patients. The macroscopically normal peritoneum tissues were collected from lateral wall left or right, near the colon ascendens or descendens.
MAIN OUTCOME MEASURE(S): The expression levels were determined as ratios between the target molecules and beta-actin as housekeeping gene.
RESULT(S): In women with endometriosis, peritoneal mRNA levels of matrix metalloproteinase (MMP)-3, transforming growth factor-beta, interleukin (IL)-6, and intercellular adhesion molecule-1 and endometrial mRNA levels of MMP-3, tumor necrosis factor (TNF)-alpha, and IL-8 were significantly higher during the menstrual phase when compared with luteal phase. During the menstrual phase of the cycle, both endometrial expression of TNF-alpha, IL-8, and MMP-3 mRNA levels and peritoneal expression of transforming growth factor-beta, IL-6, and intercellular adhesion molecule-1 mRNA levels were significantly higher in women with endometriosis when compared with controls. Immunohistochemical staining confirmed the presence of TNF-alpha in peritoneum and endometrium in both women with endometriosis and controls.
CONCLUSION(S): Increased endometrial and peritoneal cytokine mRNA expression during menstruation may contribute to a pelvic inflammatory microenvironment favoring the development of endometriosis.
研究与子宫内膜异位症患者相比，非子宫内膜异位症患者在月经周期的月经期和黄体期时，子宫内膜和腹膜中相关细胞因子、金属蛋白酶、生长因子及黏附因子的信使核糖核酸（mRNA）表达差异。
将子宫内膜异位症患者与对照患者进行比较。
大学医院。
基于月经周期阶段及是否患有子宫内膜异位症，本研究共选取了35例患者（黄体期20例，月经期15例）。
本研究中，通过腹腔镜检查和组织学检查确诊24例子宫内膜异位症患者，其中修订版美国生殖医学学会（ASRM）I-II期12例，修订版ASRM III-IV期12例；11例对照患者经腹腔镜检查证实盆腔正常。从左或右侧壁、升结肠或降结肠附近收集肉眼可见正常的腹膜组织。
将目标分子与看家基因β-肌动蛋白的表达水平以比值形式测定。
子宫内膜异位症患者中，基质金属蛋白酶（MMP）-3、转化生长因子-β、白细胞介素（IL）-6及细胞间黏附分子-1的腹膜mRNA水平，以及MMP-3、肿瘤坏死因子（TNF）-α和IL-8的子宫内膜mRNA水平在月经期显著高于黄体期。在月经周期的月经期，与对照相比，子宫内膜异位症患者的TNF-α、IL-8和MMP-3 mRNA水平的子宫内膜表达，以及转化生长因子-β、IL-6和细胞间黏附分子-1 mRNA水平的腹膜表达均显著升高。免疫组织化学染色证实，子宫内膜异位症患者和对照患者的腹膜和子宫内膜中均存在TNF-α。
月经期间子宫内膜和腹膜细胞因子mRNA表达增加，可能有助于形成有利于子宫内膜异位症发展的盆腔炎症微环境。
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