Khan Khaleque Newaz, Masuzaki Hideaki, Fujishita Akira, Kitajima Michio, Sekine Ichiro, Ishimaru Tadayuki
Department of Obstetrics and Gynecology, Nagasaki University School of Medicine, Nagasaki, Japan.
Fertil Steril. 2004 Mar;81(3):652-61. doi: 10.1016/j.fertnstert.2003.07.037.
To investigate the distribution of macrophage (Mphi) infiltration in eutopic and ectopic endometrium throughout the menstrual cycle.
Controlled clinical study using intact tissue.
Nagasaki University School of Medicine, Nagasaki, Japan.
PATIENT(S): Twenty infertile women with pelvic endometriosis and 20 women without endometriosis.
INTERVENTION(S): Biopsy specimens from peritoneal lesions and corresponding eutopic endometrium were collected from women with or without endometriosis. Adjacent peritoneal biopsies were also obtained from a fraction of these women. The activated Mphi marker CD68, mitogenic marker hepatocyte growth factor (HGF), and endothelial cell surface marker von Willebrand factor were immunolocalized and quantitated by light microscopy and Q-H score.
MAIN OUTCOME MEASURE(S): Tissue infiltration of Mphi in eutopic endometrium, ectopic endometrium, and adjacent peritoneum was examined, and its relationship with the immunoreaction of HGF and microvessel number was analyzed. The possible production of HGF by the isolated basal Mphi was also examined.
RESULT(S): Tissue infiltration of Mphi in the eutopic and ectopic endometrium of women with stage I-II endometriosis was significantly higher than with stage III-IV endometriosis or in control women. Red peritoneal lesions and their adjacent peritoneum had the greatest Mphi concentration, compared with black or white lesions. These inflammatory cells showed a higher distribution in the secretory phase of the menstrual cycle. The Mphi density in the eutopic endometrium and corresponding red lesions showed a significant correlation with both Q-H score of HGF and microvessel density. A substantial amount of HGF was also produced by the isolated basal Mphi from women with endometriosis.
CONCLUSION(S): These results suggest that the peritoneal lesions of early and active endometriosis and their adjacent peritoneum harbor abundant Mphi that could be involved in the growth of endometriosis.
研究巨噬细胞（Mphi）在整个月经周期中在位内膜和异位内膜中的浸润分布情况。
使用完整组织的对照临床研究。
日本长崎大学医学院。
20例患有盆腔子宫内膜异位症的不孕女性和20例无子宫内膜异位症的女性。
从患有或未患有子宫内膜异位症的女性中收集腹膜病变及相应在位内膜的活检标本。还从部分这些女性中获取了相邻的腹膜活检标本。通过光学显微镜和Q-H评分对活化的Mphi标志物CD68、促有丝分裂标志物肝细胞生长因子（HGF）和内皮细胞表面标志物血管性血友病因子进行免疫定位和定量分析。
检查在位内膜、异位内膜和相邻腹膜中Mphi的组织浸润情况，并分析其与HGF免疫反应及微血管数量的关系。还检测了分离的基底Mphi产生HGF的可能性。
I-II期子宫内膜异位症女性的在位和异位内膜中Mphi的组织浸润显著高于III-IV期子宫内膜异位症女性或对照女性。与黑色或白色病变相比，红色腹膜病变及其相邻腹膜的Mphi浓度最高。这些炎性细胞在月经周期的分泌期分布更高。在位内膜和相应红色病变中的Mphi密度与HGF的Q-H评分和微血管密度均呈显著相关。来自子宫内膜异位症女性的分离基底Mphi也产生大量HGF。
这些结果表明，早期和活跃期子宫内膜异位症的腹膜病变及其相邻腹膜含有丰富的Mphi，可能参与子宫内膜异位症的生长。
