Midena E, Bonaldi L, Parrozzani R, Tebaldi E, Boccassini B, Vujosevic S
Department of Ophthalmology, University of Padova, Padova and G. Bietti Eye Foundation, IRCCS, Roma--Italy.
Eur J Ophthalmol. 2006 May-Jun;16(3):422-5. doi: 10.1177/112067210601600310.
Cytogenetic prognostication of choroidal melanoma, particularly monosomy 3 detections, is limited to enucleated eyes or resected tumors. The authors developed an in vivo technique to detect monosomy 3 using transscleral fine needle aspiration biopsy (FNAB).
Eight eyes with medium-sized choroidal melanoma were included in this prospective study. A 25-gauge transscleral FNAB was performed during surgical procedure for brachytherapy, just before applying the radioactive plaque over the tumor base. Sampled material underwent fluorescence in situ hybridization (FISH) with centromeric probes for chromosome 3. Follow-up was >12 months.
Transscleral FNAB yielded sufficient material in 7 of 8 eyes (87.5 %). Five of seven eyes had monosomy 3. No early or late complications were detected.
This study demonstrates that medium choroidal melanomas may be safely sampled by intraoperative transscleral FNAB to detect monosomy 3 in vivo.
脉络膜黑色素瘤的细胞遗传学预后评估，尤其是对3号染色体单体性的检测，仅限于眼球摘除术后的眼球或切除的肿瘤。作者开发了一种利用经巩膜细针穿刺活检（FNAB）在体内检测3号染色体单体性的技术。
本前瞻性研究纳入了8只患有中等大小脉络膜黑色素瘤的眼睛。在近距离放射治疗的手术过程中，即在将放射性斑块敷于肿瘤基底之前，进行25号经巩膜FNAB。对采样材料进行3号染色体着丝粒探针的荧光原位杂交（FISH）检测。随访时间超过12个月。
8只眼中有7只（87.5%）经巩膜FNAB获得了足够的材料。7只眼中有5只存在3号染色体单体性。未检测到早期或晚期并发症。
本研究表明，术中经巩膜FNAB可安全地对中等大小的脉络膜黑色素瘤进行采样，以在体内检测3号染色体单体性。
