Les Perry A
Department of Clinical Biochemistry, St. Bartholomew's Hospital, London, UK.
Methods Mol Biol. 2006;324:125-39. doi: 10.1385/1-59259-986-9:125.
This chapter describes how adrenocorticotropin (ACTH) can be measured in biological fluids. The principle can be applied to similar peptide hormones. Following an introduction of the principle of the method, details are given on sample collection and storage, and materials required. Detailed descriptions are given for the methodology with a "Notes" section that gives practical tips on the more complex or difficult steps in the analytical process. A brief bibliography lists peer reviewed articles relevant to the development and application of this method.
本章描述了如何在生物体液中测量促肾上腺皮质激素(ACTH)。该原理可应用于类似的肽类激素。在介绍该方法的原理之后,详细说明了样本采集与保存以及所需材料。对该方法进行了详细描述,并设有“注释”部分,针对分析过程中较为复杂或困难的步骤给出实用提示。一份简短的参考文献列表列出了与该方法的开发和应用相关的同行评审文章。