实时荧光定量PCR检测NK/T细胞淋巴瘤中核糖体蛋白S13（RPS13）基因表达
[Real-time fluorescence quantitative PCR in detecting ribosome protein S13 (RPS13) gene expression in NK/T cell lymphoma].
作者信息
Yang Fan, Liu Wei-ping, He Miao-xia, Tang Qiong-lan, Zhao Sha, Zhang Wen-yan, Xia Qing-jie, Li Gan-di
机构信息
Department of Pathology, West China Hospital, Sichuan University, Chengdu 610041, China.
出版信息
Sichuan Da Xue Xue Bao Yi Xue Ban. 2006 May;37(3):464-6.
OBJECTIVE
To quantitatively detect the expression level of ribosome protein S13 (RPS13) in NK/T cell lymphoma.
METHODS
The total RNA isolated from the parafin-embedded tissue of NK/T cell lymphoma was reversely transcribed into cDNA. The real-time fluorescence quantitative PCR technology method was used to analyze the expression level of RPS13 gene.
RESULTS
The real-time fluorescence quantitative PCR technology was successfully performed to precisely detect the mRNA level. The expression level of RPS13 gene in tumor tissue of 20 cases of NK/T cell lymphoma was dramatically lower than that in normal peripheral blood lymphocyte of healthy controls.
CONCLUSION
The RPS13 gene has lower expression level in tumor tissue cells of NK/T cell lymphoma than in normal lymphocyte, indicating that it plays an role in the development of the NK/T cell lymphoma.
目的
定量检测核糖体蛋白S13（RPS13）在NK/T细胞淋巴瘤中的表达水平。
方法
从NK/T细胞淋巴瘤石蜡包埋组织中提取的总RNA逆转录成cDNA。采用实时荧光定量PCR技术方法分析RPS13基因的表达水平。
结果
成功进行实时荧光定量PCR技术以精确检测mRNA水平。20例NK/T细胞淋巴瘤肿瘤组织中RPS13基因的表达水平显著低于健康对照者的正常外周血淋巴细胞。
结论
RPS13基因在NK/T细胞淋巴瘤肿瘤组织细胞中的表达水平低于正常淋巴细胞，表明其在NK/T细胞淋巴瘤的发生发展中起作用。
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