[毛细管气相色谱法测定保健食品中的甘露醇]
[Determination of mannitol in health food by capillary gas chromatography].
作者信息
Wang He-xing, Li Yuan-qian, Li Lei
机构信息
Department of Sanitary Technology, West China School of Public Health, Sichuan University, Chengdu 610041, China.
出版信息
Sichuan Da Xue Xue Bao Yi Xue Ban. 2006 May;37(3):480-3.
OBJECTIVE
To develop a capillary gas chromatographic method for the determination of mannitol in health food.
METHODS
Mannitol was extracted from the sample with water by ultrasonic wave. The aliquot of sample solution was acetylated to form mannitol hexacetate with lower boiling point. Then the derivative was separated with the use of SPB-608 capillary chromatographic column and determined by flame ionization detector (FID) at the column temperature of 180 degrees C, the detector temperature of 210 degrees C and the nitrogen flow rate of 30 mL/min.
RESULTS
The linear range of this method was 0.05-10.00 mg/mL and the limit of detection of was 0.01 mg/mL. The within-day and between-days precisions were 4.4% and 7.3%-7.6%, respectively. The average recoveries were 84.6%-107.6%. Mannitol could be separated from glucose, inositol and sorbitol under the optimum chromatographic condition.
CONCLUSION
The results demonstrate that the method has the advantages of simplicity, accuracy and sensitivity. It is suitable for the determination of mannitol in health food.
目的
建立一种毛细管气相色谱法测定保健食品中的甘露醇。
方法
用水超声提取样品中的甘露醇。取适量样品溶液进行乙酰化反应，生成沸点较低的甘露醇六乙酸酯。然后使用SPB - 608毛细管色谱柱分离衍生物，采用火焰离子化检测器（FID）在柱温180℃、检测器温度210℃、氮气流量30 mL/min的条件下进行测定。
结果
该方法的线性范围为0.05 - 10.00 mg/mL，检测限为0.01 mg/mL。日内精密度和日间精密度分别为4.4%和7.3% - 7.6%。平均回收率为84.6% - 107.6%。在最佳色谱条件下，甘露醇可与葡萄糖、肌醇和山梨醇分离。
结论
结果表明该方法具有简便、准确、灵敏的优点。适用于保健食品中甘露醇的测定。
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