一种用脱氧核糖核酸酶1切割染色质以进行超敏位点分析的更简单且更好的方法。 - Suppr

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A simpler and better method to cleave chromatin with DNase 1 for hypersensitive site analyses.

作者信息

Stewart A F, Reik A, Schütz G

机构信息

German Cancer Research Center, Heidelberg.

出版信息

Nucleic Acids Res. 1991 Jun 11;19(11):3157. doi: 10.1093/nar/19.11.3157.

DOI:10.1093/nar/19.11.3157

PMID:1676154

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC328292/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a596/328292/38de494c6466/nar00091-0330-a.jpg

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A simpler and better method to cleave chromatin with DNase 1 for hypersensitive site analyses.

Nucleic Acids Res. 1991 Jun 11;19(11):3157. doi: 10.1093/nar/19.11.3157.

Transformation-induced alterations in the regulation of tyrosine aminotransferase expression in fetal rat hepatocytes: changes in hormone inducibility and the DNase-hypersensitive site.

Cancer Res. 1993 Feb 1;53(3):515-22.

Glucocorticoids locally disrupt an array of positioned nucleosomes on the rat tyrosine aminotransferase promoter in hepatoma cells.

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[DNA-protein interactions in CpG islands and DNAse I sensitive chromatin].

Biull Eksp Biol Med. 1992 May;113(5):491-3.

Cell specific differences in DNase I hypersensitivity between the two promoters of the rat glucokinase gene.

Biochem Biophys Res Commun. 1995 Oct 4;215(1):272-9. doi: 10.1006/bbrc.1995.2462.

DNase I and hydroxyl radical characterization of chromatin complexes.

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[Intranuclear localization of hepatoma chromatin antigens not detected in liver chromatin].

Eksp Onkol. 1984;6(5):39-42.

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The non-histone proteins of the rat liver nucleus and their distribution amongst chromatin fractions as produced by nuclease digestion.

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A simpler and better method to cleave chromatin with DNase 1 for hypersensitive site analyses.

作者信息

Stewart A F, Reik A, Schütz G

机构信息

German Cancer Research Center, Heidelberg.

出版信息

Nucleic Acids Res. 1991 Jun 11;19(11):3157. doi: 10.1093/nar/19.11.3157.

DOI:10.1093/nar/19.11.3157

PMID:1676154

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC328292/

Abstract

摘要

相似文献

A simpler and better method to cleave chromatin with DNase 1 for hypersensitive site analyses.

Nucleic Acids Res. 1991 Jun 11;19(11):3157. doi: 10.1093/nar/19.11.3157.

Transformation-induced alterations in the regulation of tyrosine aminotransferase expression in fetal rat hepatocytes: changes in hormone inducibility and the DNase-hypersensitive site.

Cancer Res. 1993 Feb 1;53(3):515-22.

Glucocorticoids locally disrupt an array of positioned nucleosomes on the rat tyrosine aminotransferase promoter in hepatoma cells.

Proc Natl Acad Sci U S A. 1990 Dec;87(23):9300-4. doi: 10.1073/pnas.87.23.9300.

Preferential binding of adriamycin and nogalamycin to DNase-I hypersensitive sites of Sarcoma-180 chromatin.

Chem Biol Interact. 1986 Jan;57(1):65-72. doi: 10.1016/0009-2797(86)90049-9.

Requirement of distal and proximal promoter sequences for chromatin organization of the osteocalcin gene in bone-derived cells.

J Cell Biochem. 1996 Nov 1;63(2):221-8. doi: 10.1002/(SICI)1097-4644(19961101)63:2%3C221::AID-JCB9%3E3.0.CO;2-#.

[DNA-protein interactions in CpG islands and DNAse I sensitive chromatin].

Biull Eksp Biol Med. 1992 May;113(5):491-3.

Cell specific differences in DNase I hypersensitivity between the two promoters of the rat glucokinase gene.

Biochem Biophys Res Commun. 1995 Oct 4;215(1):272-9. doi: 10.1006/bbrc.1995.2462.

DNase I and hydroxyl radical characterization of chromatin complexes.

Curr Protoc Mol Biol. 2001 May;Chapter 21:Unit 21.4. doi: 10.1002/0471142727.mb2104s48.

[Intranuclear localization of hepatoma chromatin antigens not detected in liver chromatin].

Eksp Onkol. 1984;6(5):39-42.

Selective loss of a DNase I hypersensitive site upstream of the tyrosine aminotransferase gene in mice homozygous for lethal albino deletions.

Proc Natl Acad Sci U S A. 1992 Jul 15;89(14):6540-4. doi: 10.1073/pnas.89.14.6540.

引用本文的文献

Nucleosome dynamics of human iPSC during neural differentiation.

EMBO Rep. 2019 Jun;20(6). doi: 10.15252/embr.201846960. Epub 2019 Apr 29.

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Nucleic Acids Res. 2019 Feb 20;47(3):1239-1254. doi: 10.1093/nar/gky1203.

High-resolution nucleosome mapping of targeted regions using BAC-based enrichment.

Nucleic Acids Res. 2013 Apr;41(7):e87. doi: 10.1093/nar/gkt081. Epub 2013 Feb 14.

Rapid genome-scale mapping of chromatin accessibility in tissue.

Epigenetics Chromatin. 2012 Jun 26;5(1):10. doi: 10.1186/1756-8935-5-10.

Leukemic transformation by the v-ErbA oncoprotein entails constitutive binding to and repression of an erythroid enhancer in vivo.

EMBO J. 1998 Dec 15;17(24):7382-94. doi: 10.1093/emboj/17.24.7382.

Chromatin remodeling of the interleukin-2 gene: distinct alterations in the proximal versus distal enhancer regions.

Nucleic Acids Res. 1998 Jun 15;26(12):2923-34. doi: 10.1093/nar/26.12.2923.

A rapid method for chromatin structure analysis in the filamentous fungus Aspergillus nidulans.

Nucleic Acids Res. 1997 Oct 1;25(19):3955-6. doi: 10.1093/nar/25.19.3955.

The centromere and promoter factor, 1, CPF1, of Saccharomyces cerevisiae modulates gene activity through a family of factors including SPT21, RPD1 (SIN3), RPD3 and CCR4.

Mol Gen Genet. 1993 Sep;240(3):374-86. doi: 10.1007/BF00280389.

Chromatin analysis in yeast using NP-40 permeabilised sphaeroplasts.

Nucleic Acids Res. 1993 Sep 25;21(19):4653-4. doi: 10.1093/nar/21.19.4653.

Improved preservation of chromatin structure in ethanol-fixed cells.

Nucleic Acids Res. 1994 May 25;22(10):1928-9. doi: 10.1093/nar/22.10.1928.

本文引用的文献

Tissue-specific DNaseI hypersensitive sites in the 5'-flanking sequences of the tryptophan oxygenase and the tyrosine aminotransferase genes.

EMBO J. 1984 Sep;3(9):2015-20. doi: 10.1002/j.1460-2075.1984.tb02084.x.

Camptothecin-induced in vivo topoisomerase I cleavages in the transcriptionally active tyrosine aminotransferase gene.

Cell. 1987 Sep 25;50(7):1109-17. doi: 10.1016/0092-8674(87)90177-2.

In situ nucleoprotein structure involving origin-proximal SV40 DNA control elements.

Nucleic Acids Res. 1990 Apr 11;18(7):1797-803. doi: 10.1093/nar/18.7.1797.

The non-histone proteins of the rat liver nucleus and their distribution amongst chromatin fractions as produced by nuclease digestion.

Nucleic Acids Res. 1979 Sep 11;7(1):31-48. doi: 10.1093/nar/7.1.31.