Sachdeva N, Sehgal S, Sud A, Datta U, Arora S K
Department of Immunopathology, Postgraduate Institute of Medical Education & Research, Chandigarh.
Indian J Pathol Microbiol. 2005 Jul;48(3):337-40.
Screening of drug-resistant variants is very important for the effective clinical management of HIV-infected patients and development of new strategies. The present study was aimed to detect codon-184 mutations in the pol-gene of HIV leading to resistance to lamivudine (3-TC) by nested cum ARMS-PCR approach in 10 treated and 9 treatment naive patients. For correlation the whole blood CD4/CD8 cell counts and the soluble TNFRII levels in plasma were also determined. Of the 19 patients tested, mutant variants were observed in 2 patients (Met Val in one and Met Val & lle in second) both being treated with 3-TC. No mutations were detected in the treatment-naive patients. The results confirmed that, drug resistant variants of codon-184 emerge rapidly in patients receiving 3-TC containing regimens including our population, which is mainly infected with subtypeC of the virus that could be detected along with wild viral population using sensitive approaches such as ARMS-PCR.
耐药变异体的筛查对于HIV感染患者的有效临床管理和新策略的开发非常重要。本研究旨在通过巢式累积ARMS-PCR方法，检测10例接受治疗的患者和9例未接受过治疗的患者中，HIV pol基因导致对拉米夫定（3-TC）耐药的184位密码子突变。为了进行相关性分析，还测定了全血CD4/CD8细胞计数和血浆中可溶性TNFRII水平。在19例接受检测的患者中，2例接受3-TC治疗的患者检测到突变变异体（1例为Met Val，另1例为Met Val和Ile）。未接受过治疗的患者未检测到突变。结果证实，在接受含3-TC治疗方案的患者中，包括我们的研究人群（主要感染病毒C亚型），184位密码子的耐药变异体迅速出现，使用ARMS-PCR等敏感方法可与野生病毒群体一起检测到这些变异体。
