雌激素受体α基因多态性与妊娠期肝内胆汁淤积症的相关性研究

[Study on the association between estrogen receptor alpha gene polymorphism and intrahepatic cholestasis of pregnancy].

作者信息

Zhang Li, Liu Shu-yun, Shi Qing-yun, Chen Qiang, Zou Hai, Xing Ai-yun

机构信息

Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, China.

出版信息

Zhonghua Fu Chan Ke Za Zhi. 2006 May;41(5):307-10.

PMID:16762184

Abstract

OBJECTIVE

To investigate the relationship between estrogen receptor alpha (ERalpha) gene polymorphism and intrahepatic cholestasis of pregnancy (ICP).

METHODS

The Xba I and Pvu II polymorphisms in intron 1 of ER alpha gene were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in 100 pregnant women with ICP (ICP group) and 100 normal pregnant women (control group).

RESULTS

(1) The frequencies of XX, Xx and xx genotypes of Xba I polymorphism were 6%, 33% and 61% respectively in control group, and were 2%, 33% and 65% respectively in ICP group. There was no significant difference in the distribution of Xba I genotypes between the two groups (P > 0.05). The frequencies of the two alleles X and x were 23% and 78% in control group, and were 19% and 82% in ICP group, respectively. There was also no significant difference in the frequency of Xba I alleles between the two groups (P > 0.05). (2) The frequencies of PP, Pp and pp genotypes of Pvu II polymorphism were 18%, 42% and 40% respectively in control group, and were 12%, 53% and 35% respectively in ICP group. There was no significant difference in the distribution of Pvu II genotypes between the two groups (P > 0.05). The frequencies of the two alleles P and p were 39% and 61% in control group, and were 39% and 62% in ICP group, respectively. There was also no significant difference in the frequency of Pvu II alleles between the two groups (P > 0.05). (3) There was also no significant difference in the distribution of Xba I and Pvu II combinative genotype between the two groups (P > 0.05).

CONCLUSION

The ERalpha gene polymorphism is not associated with the risk of ICP.

摘要

目的

探讨雌激素受体α（ERα）基因多态性与妊娠期肝内胆汁淤积症（ICP）的关系。

方法

采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法，检测100例ICP孕妇（ICP组）和100例正常孕妇（对照组）ERα基因第1内含子的Xba I和Pvu II多态性。

结果

（1）对照组Xba I多态性XX、Xx和xx基因型频率分别为6%、33%和61%，ICP组分别为2%、33%和65%。两组间Xba I基因型分布无显著差异（P>0.05）。对照组X和x两个等位基因频率分别为23%和78%，ICP组分别为19%和82%。两组间Xba I等位基因频率也无显著差异（P>0.05）。（2）对照组Pvu II多态性PP、Pp和pp基因型频率分别为18%、42%和40%，ICP组分别为12%、53%和35%。两组间Pvu II基因型分布无显著差异（P>0.05）。对照组P和p两个等位基因频率分别为39%和61%，ICP组分别为39%和62%。两组间Pvu II等位基因频率也无显著差异（P>0.05）。（3）两组间Xba I和Pvu II联合基因型分布也无显著差异（P>0.05）。