[通过RNA干扰卵巢癌细胞系中的切除修复交叉互补1基因增强顺铂细胞毒性]
[Enhanced cisplatin cytotoxicity by RNA interfering the excision repair cross-complementing 1 gene in ovarian cancer cell lines].
作者信息
Liu Guo-yan, Qu Quan-xin, Mi Ruo-ran, Qi Jing
机构信息
Department of Obstetrics and Gynecology, General Hospital of Tianjin Medical University, Tianjin 300052, China.
出版信息
Zhonghua Fu Chan Ke Za Zhi. 2006 May;41(5):339-42.
OBJECTIVE
To study changes of cisplatin sensitivity by RNA interfering the excision repair cross-complementing (ERCC) 1 gene in ovarian cancer cell lines.
METHODS
The small interference RNA (siRNA) targeting ERCC1 gene was designed and synthesized by transcription in vitro, and transfected to ovarian cancer cell line ES-2. The mRNA and protein of ERCC1 were evaluated by means of RT-PCR, western blot and immunocytochemistry. The changes of cisplatin sensitivity after interference were examined by methyl thiazolyl tetrazolium (MTT) assay.
RESULTS
In ES-2 cell, the mRNA and protein levels of ERCC1 were dramatically decreased 24, 48 and 72 hours after transfection. The sensitivity to cisplatin of ES-2 cell line was increased by 53.88 times after disturbing the ERCC1 gene.
CONCLUSION
The sensitivity to cisplatin of ovarian cancer cell lines ES-2 could be enhanced by RNA interfering ERCC1 gene.
目的
通过RNA干扰卵巢癌细胞系中的切除修复交叉互补（ERCC）1基因，研究顺铂敏感性的变化。
方法
设计并体外转录合成靶向ERCC1基因的小干扰RNA（siRNA），转染至卵巢癌细胞系ES-2。采用逆转录聚合酶链反应（RT-PCR）、蛋白质免疫印迹法（western blot）及免疫细胞化学法检测ERCC1的mRNA和蛋白。采用甲基噻唑基四氮唑（MTT）法检测干扰后顺铂敏感性的变化。
结果
在ES-2细胞中，转染后24、48和72小时，ERCC1的mRNA和蛋白水平显著降低。干扰ERCC1基因后，ES-2细胞系对顺铂的敏感性提高了53.88倍。
结论
RNA干扰ERCC1基因可增强卵巢癌细胞系ES-2对顺铂的敏感性。
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