Liu Guo-Yan, Qu Quan-Xin, Mi Ruo-Ran, Qi Jing
Department of Obstetrics and Gynecology, General Hospital of Tianjin Medical University, Tianjin 300052, China.
Zhonghua Zhong Liu Za Zhi. 2008 Mar;30(3):184-7.
To study the relationship between nucleotide excision repair gene ERCC1 and resistance to cisplatin in ovarian cancer.
The expression of gene ERCC1 in 58 ovarian cancer tissues and 4 cell lines were examined and its relationship with resistance to cisplatin were analyzed, the changes of sensitivity to cisplatin were observed after interference of ERCC1 gene with small interfering RNA (siRNA) in ovarian cancer cell lines.
In 58 ovarian cancer tissues, the positive rate of ERCC1 protein in chemoresistant cases (57.89%) was higher than that in chemo-sensitive cases (28.21%, P = 0.029). The mRNA levels of ERCC1 gene in ovarian cancer cell lines ES-2, SKOV3, COC1, COC1/DDP were related to cisplatin IC50 values (r = 0.932, P <0.05). The sensitivity of cell lines ES-2, SKOV3, COC1/DDP cells to cisplatin was increased by 53.88, 5.07, and 3.75 times, respectively, after RNA interfering ERCC1 gene.
ERCC1 gene is associated with the resistance to cisplatin and the sensitivity to cisplatin can be enhanced by RNA interfering ERCC1 in ovarian cancer.
研究核苷酸切除修复基因ERCC1与卵巢癌顺铂耐药性之间的关系。
检测58例卵巢癌组织及4种细胞系中ERCC1基因的表达情况，并分析其与顺铂耐药性的关系；在卵巢癌细胞系中采用小干扰RNA（siRNA）干扰ERCC1基因后，观察顺铂敏感性的变化。
58例卵巢癌组织中，化疗耐药病例的ERCC1蛋白阳性率（57.89%）高于化疗敏感病例（28.21%，P = 0.029）。卵巢癌细胞系ES-2、SKOV3、COC1、COC1/DDP中ERCC1基因的mRNA水平与顺铂IC50值相关（r = 0.932，P <0.05）。RNA干扰ERCC1基因后，细胞系ES-2、SKOV3、COC1/DDP对顺铂的敏感性分别提高了53.88倍、5.07倍和3.75倍。
ERCC1基因与卵巢癌顺铂耐药相关，RNA干扰ERCC1可增强卵巢癌对顺铂的敏感性。
