Zhang Hong, Zhao Guo-hong, Zhang Qi, Dong Xue-chuan, Hu Wei-kun, Xie Er-juan, Li Gui-gang
Department of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, China.
Zhonghua Yan Ke Za Zhi. 2006 May;42(5):431-4.
To measure the number of glucocorticoid receptors (GR) in the trabecular meshwork and peripheral blood lymphocytes of rabbits, and to explore their relationship with steroid-induced glaucoma.
Steroid-induced glaucoma model was induced by subconjunctival injection of 0.5 mg dexamethasone in the right eyes every two days for a month. Before the injection, GR concentration was measured in peripheral blood lymphocytes and trabecular meshwork of rabbits using the radio-ligand binding assay. GR concentration was measured again 30 days after the injection. The data was analyzed with SPSS software.
The GR concentration in the peripheral blood lymphocytes and the trabecular meshwork of rabbits was (3642 +/- 947) site/cell and (2437.85 +/- 733.93) dmp/mg, respectively. The expression level in lymphocytes was significantly correlated with that in the trabecular (r = 0.862, P < 0.01). After treated with dexamethasone, the intraocular pressure of rabbits increased significantly, and the GR concentration in peripheral blood lymphocytes decreased significantly. Elevating level of IOP correlated positively with the primary and decreasing level of GR (r = 0.78, 0.79, P < 0.01).
GR concentration in the peripheral blood lymphocytes of rabbits can reflect directly the GR number in the trabecular meshwork. There may be a close relationship between the GR number in peripheral blood lymphocytes and steroid-induced glaucoma.
测量兔小梁网和外周血淋巴细胞中糖皮质激素受体（GR）的数量，并探讨它们与类固醇性青光眼的关系。
通过每两天在右眼结膜下注射0.5mg地塞米松，持续一个月，诱导建立类固醇性青光眼模型。注射前，采用放射性配体结合分析法测量兔外周血淋巴细胞和小梁网中的GR浓度。注射30天后再次测量GR浓度。数据用SPSS软件进行分析。
兔外周血淋巴细胞和小梁网中的GR浓度分别为（3642±947）位点/细胞和（2437.85±733.93）dmp/mg。淋巴细胞中的表达水平与小梁网中的表达水平显著相关（r = 0.862，P < 0.01）。用地塞米松治疗后，兔眼压显著升高，外周血淋巴细胞中的GR浓度显著降低。眼压升高水平与GR的初始水平和降低水平呈正相关（r = 0.78，0.79，P < 0.01）。
兔外周血淋巴细胞中的GR浓度可直接反映小梁网中的GR数量。外周血淋巴细胞中的GR数量与类固醇性青光眼之间可能存在密切关系。
